Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Here we describe a method to conditionally induce epithelial cell transformation by the use of the 4-Hydroxytamoxifen (4-OHT) inducible KalTA4-ER(T2)/UAS expression system(1) in zebrafish larvae, and the subsequent live imaging of innate immune cell interaction with HRAS(G12V) expressing skin cells....

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Detalles Bibliográficos
Autores principales: Ramezani, Thomas, Laux, Derek W., Bravo, Isabel R., Tada, Masazumi, Feng, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354608/
https://www.ncbi.nlm.nih.gov/pubmed/25741625
http://dx.doi.org/10.3791/52107

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