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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
The ability to study biomolecules in vivo is crucial for understanding their function in a biological context. One powerful approach involves fusing molecules of interest to fluorescent proteins such as GFP to study their expression, localization and function. However, GFP and its derivatives are si...
Autores principales: | Aigrain, Louise, Sustarsic, Marko, Crawford, Robert, Plochowietz, Anne, Kapanidis, Achillefs N. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354625/ https://www.ncbi.nlm.nih.gov/pubmed/25741968 http://dx.doi.org/10.3791/52208 |
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