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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

The ability to study biomolecules in vivo is crucial for understanding their function in a biological context. One powerful approach involves fusing molecules of interest to fluorescent proteins such as GFP to study their expression, localization and function. However, GFP and its derivatives are si...

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Detalles Bibliográficos
Autores principales: Aigrain, Louise, Sustarsic, Marko, Crawford, Robert, Plochowietz, Anne, Kapanidis, Achillefs N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354625/
https://www.ncbi.nlm.nih.gov/pubmed/25741968
http://dx.doi.org/10.3791/52208

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