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Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China
BACKGROUND: Hainan Province is one of the most severe endemic regions with high transmission of Plasmodium falciparum and Plasmodium vivax in China. However, the incidence of P. falciparum and P. vivax has dropped dramatically since 2007 and a national elimination malaria programme (NEMP) was launch...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354742/ https://www.ncbi.nlm.nih.gov/pubmed/25888891 http://dx.doi.org/10.1186/s12936-015-0545-2 |
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author | Li, Yu-Chun Wang, Guang-Ze Meng, Feng Zeng, Wen He, Chang-hua Hu, Xi-Min Wang, Shan-Qing |
author_facet | Li, Yu-Chun Wang, Guang-Ze Meng, Feng Zeng, Wen He, Chang-hua Hu, Xi-Min Wang, Shan-Qing |
author_sort | Li, Yu-Chun |
collection | PubMed |
description | BACKGROUND: Hainan Province is one of the most severe endemic regions with high transmission of Plasmodium falciparum and Plasmodium vivax in China. However, the incidence of P. falciparum and P. vivax has dropped dramatically since 2007 and a national elimination malaria programme (NEMP) was launched after 2010. To better understand the genetic information on P. vivax population before elimination of malaria in Hainan Province, the extent of genetic diversity of P. vivax isolates in Hainan Province was investigated using four polymorphic genetic markers, including P. vivax merozoite surface proteins 1, 3α, and 3β (pvmsp-1, pvmsp-3α, and pvmsp-3β) and circumsporozoite protein (pvcsp). METHODS: Isolates of P. vivax (n = 27) from Hainan Province were collected from 2009 to 2010 and pvmsp-1 and pvcsp were analysed by DNA sequencing, respectively. Using polymerase chain reaction-restriction fragment length polymorphism were analysed in pvmsp-3α, and pvmsp-3β. RESULTS: The DNA sequencing analysis on pvmsp1 revealed that there were three allele types: Salvador-1 (Sal-1), Belem and recombinant (R) types. Among them, Sal-1 type was a dominant strain with eight variant subtypes (88.9%), whereas R- (3.7%) and Belem-type strains (7.4%) had one variant subtypes, respectively. All the isolates carried pvcsp with VK210 type accounting for 85.2% (23/27 isolates) and VK247 type accounting for 14.8% (4/27). Only type A and type B alleles were successfully amplified in pvmsp-3α gene, and a high level of polymorphism was observed in pvmsp-3α. Considering pvmsp-3β gene, type A was the predominant type in 17 isolates (63%), whereas type B was dominant in only ten isolates (37%). CONCLUSION: The present data indicate that there was high degree of genetic diversity among P. vivax population in Hainan Province of China during the pre-elimination stage of malaria, with 26 unique haplotypes observed among 27 samples. |
format | Online Article Text |
id | pubmed-4354742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43547422015-03-11 Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China Li, Yu-Chun Wang, Guang-Ze Meng, Feng Zeng, Wen He, Chang-hua Hu, Xi-Min Wang, Shan-Qing Malar J Research BACKGROUND: Hainan Province is one of the most severe endemic regions with high transmission of Plasmodium falciparum and Plasmodium vivax in China. However, the incidence of P. falciparum and P. vivax has dropped dramatically since 2007 and a national elimination malaria programme (NEMP) was launched after 2010. To better understand the genetic information on P. vivax population before elimination of malaria in Hainan Province, the extent of genetic diversity of P. vivax isolates in Hainan Province was investigated using four polymorphic genetic markers, including P. vivax merozoite surface proteins 1, 3α, and 3β (pvmsp-1, pvmsp-3α, and pvmsp-3β) and circumsporozoite protein (pvcsp). METHODS: Isolates of P. vivax (n = 27) from Hainan Province were collected from 2009 to 2010 and pvmsp-1 and pvcsp were analysed by DNA sequencing, respectively. Using polymerase chain reaction-restriction fragment length polymorphism were analysed in pvmsp-3α, and pvmsp-3β. RESULTS: The DNA sequencing analysis on pvmsp1 revealed that there were three allele types: Salvador-1 (Sal-1), Belem and recombinant (R) types. Among them, Sal-1 type was a dominant strain with eight variant subtypes (88.9%), whereas R- (3.7%) and Belem-type strains (7.4%) had one variant subtypes, respectively. All the isolates carried pvcsp with VK210 type accounting for 85.2% (23/27 isolates) and VK247 type accounting for 14.8% (4/27). Only type A and type B alleles were successfully amplified in pvmsp-3α gene, and a high level of polymorphism was observed in pvmsp-3α. Considering pvmsp-3β gene, type A was the predominant type in 17 isolates (63%), whereas type B was dominant in only ten isolates (37%). CONCLUSION: The present data indicate that there was high degree of genetic diversity among P. vivax population in Hainan Province of China during the pre-elimination stage of malaria, with 26 unique haplotypes observed among 27 samples. BioMed Central 2015-02-14 /pmc/articles/PMC4354742/ /pubmed/25888891 http://dx.doi.org/10.1186/s12936-015-0545-2 Text en © Li et al.; licensee Biomed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Yu-Chun Wang, Guang-Ze Meng, Feng Zeng, Wen He, Chang-hua Hu, Xi-Min Wang, Shan-Qing Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China |
title | Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China |
title_full | Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China |
title_fullStr | Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China |
title_full_unstemmed | Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China |
title_short | Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China |
title_sort | genetic diversity of plasmodium vivax population before elimination of malaria in hainan province, china |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354742/ https://www.ncbi.nlm.nih.gov/pubmed/25888891 http://dx.doi.org/10.1186/s12936-015-0545-2 |
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