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Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo
The use of phytochemicals has been widely used as inexpensive approach for prevention of diseases related to oxidative damage due to its antioxidant properties. One of dietary flavonoids is chrysin (CR), found mainly in passion fruit, honey, and propolis. Methylmercury (MeHg) is a toxic metal whose...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355113/ https://www.ncbi.nlm.nih.gov/pubmed/25810809 http://dx.doi.org/10.1155/2015/602360 |
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author | Manzolli, Eduardo Scandinari Serpeloni, Juliana Mara Grotto, Denise Bastos, Jairo Kennup Antunes, Lusânia Maria Greggi Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron |
author_facet | Manzolli, Eduardo Scandinari Serpeloni, Juliana Mara Grotto, Denise Bastos, Jairo Kennup Antunes, Lusânia Maria Greggi Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron |
author_sort | Manzolli, Eduardo Scandinari |
collection | PubMed |
description | The use of phytochemicals has been widely used as inexpensive approach for prevention of diseases related to oxidative damage due to its antioxidant properties. One of dietary flavonoids is chrysin (CR), found mainly in passion fruit, honey, and propolis. Methylmercury (MeHg) is a toxic metal whose main toxic mechanism is oxidative damage. Thus, the study aimed to evaluate the antioxidant effects of CR against oxidative damage induced by MeHg in Wistar rats. Animals were treated with MeHg (30 µg/kg/bw) in presence and absence of CR (0.10, 1.0, and 10 mg/kg/bw) by gavage for 45 days. Glutathione (GSH) in blood was quantified spectrophotometrically and for monitoring of DNA damage, comet assay was used in leukocytes and hepatocytes. MeHg led to a significant increase in the formation of comets; when the animals were exposed to the metal in the presence of CR, higher concentrations of CR showed protective effects. Moreover, exposure to MeHg decreased the levels of GSH and GSH levels were restored in the animals that received CR plus MeHg. Taken together the findings of the present work indicate that consumption of flavonoids such as CR may protect humans against the adverse health effects caused by MeHg. |
format | Online Article Text |
id | pubmed-4355113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43551132015-03-25 Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo Manzolli, Eduardo Scandinari Serpeloni, Juliana Mara Grotto, Denise Bastos, Jairo Kennup Antunes, Lusânia Maria Greggi Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron Oxid Med Cell Longev Research Article The use of phytochemicals has been widely used as inexpensive approach for prevention of diseases related to oxidative damage due to its antioxidant properties. One of dietary flavonoids is chrysin (CR), found mainly in passion fruit, honey, and propolis. Methylmercury (MeHg) is a toxic metal whose main toxic mechanism is oxidative damage. Thus, the study aimed to evaluate the antioxidant effects of CR against oxidative damage induced by MeHg in Wistar rats. Animals were treated with MeHg (30 µg/kg/bw) in presence and absence of CR (0.10, 1.0, and 10 mg/kg/bw) by gavage for 45 days. Glutathione (GSH) in blood was quantified spectrophotometrically and for monitoring of DNA damage, comet assay was used in leukocytes and hepatocytes. MeHg led to a significant increase in the formation of comets; when the animals were exposed to the metal in the presence of CR, higher concentrations of CR showed protective effects. Moreover, exposure to MeHg decreased the levels of GSH and GSH levels were restored in the animals that received CR plus MeHg. Taken together the findings of the present work indicate that consumption of flavonoids such as CR may protect humans against the adverse health effects caused by MeHg. Hindawi Publishing Corporation 2015 2015-02-24 /pmc/articles/PMC4355113/ /pubmed/25810809 http://dx.doi.org/10.1155/2015/602360 Text en Copyright © 2015 Eduardo Scandinari Manzolli et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Manzolli, Eduardo Scandinari Serpeloni, Juliana Mara Grotto, Denise Bastos, Jairo Kennup Antunes, Lusânia Maria Greggi Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo |
title | Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo
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title_full | Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo
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title_fullStr | Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo
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title_full_unstemmed | Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo
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title_short | Protective Effects of the Flavonoid Chrysin against Methylmercury-Induced Genotoxicity and Alterations of Antioxidant Status, In Vivo
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title_sort | protective effects of the flavonoid chrysin against methylmercury-induced genotoxicity and alterations of antioxidant status, in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355113/ https://www.ncbi.nlm.nih.gov/pubmed/25810809 http://dx.doi.org/10.1155/2015/602360 |
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