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Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area

BACKGROUND: Illegal commercial plasma and blood donation activities in the late 1980s and early 1990s caused a large number of hepatitis C virus (HCV) infections in rural areas of China. In the present study, we aimed to elucidate the risk factors of HCV RNA positivity and HCV genotype distribution...

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Autores principales: Yin, Wenjiao, Huang, Changhong, Qiu, Feng, Liu, Li, Wang, Feng, Zhou, Jikun, Zhang, Yong, Bi, Shengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355568/
https://www.ncbi.nlm.nih.gov/pubmed/25884321
http://dx.doi.org/10.1186/s12889-015-1535-6
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author Yin, Wenjiao
Huang, Changhong
Qiu, Feng
Liu, Li
Wang, Feng
Zhou, Jikun
Zhang, Yong
Bi, Shengli
author_facet Yin, Wenjiao
Huang, Changhong
Qiu, Feng
Liu, Li
Wang, Feng
Zhou, Jikun
Zhang, Yong
Bi, Shengli
author_sort Yin, Wenjiao
collection PubMed
description BACKGROUND: Illegal commercial plasma and blood donation activities in the late 1980s and early 1990s caused a large number of hepatitis C virus (HCV) infections in rural areas of China. In the present study, we aimed to elucidate the risk factors of HCV RNA positivity and HCV genotype distribution in former blood donors. METHODS: A cross-sectional survey was carried out in a former blood donation village in rural Hebei Province, North China. All residents were invited for a questionnaire interview and testing for HCV antibodies as well as HCV nucleic acids. Questionnaires were administered to collect information about their personal status and commercial blood donation history. Nested PCR was used to amplify HCV nucleic acids in C/E1 region and NS5b region followed by genotyping and phylogenetic analysis. Univariate and multivariate logistic regression were used to analyze the distributions of HCV genotypes in different groups. RESULTS: A total of 512 blood samples were collected. Anti-HCV positive were 148 (28.5%) whereas RNA positive rate was 13.87%. Residents between 50 and 59 years old had the highest RNA positive rate (27/109, 24.77%) (P = 0.0051). Multivariate logistic regression model analysis revealed that plasma donation (OR = 8.666, 95% CI: 1.390-54.025) was the dominant risk factor of HCV infection. Furthermore, HCV subtypes 1b and 2a were found by genotyping and phylogenetic analysis. 36 samples (53.73%) were subtype 1b and 31 samples (46.27%) were subtype 2a. CONCLUSIONS: Unsafe practices during illegal plasma donation led to a high risk of HCV infection. The identification of genotypes 1b and 2a as major HCV genotypes circulating in this region may help to predict the future burden of HCV related diseases and facilitate better medical treatment towards HCV carriers. These results are useful for public healthcare as well as disease control and surveillance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12889-015-1535-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-43555682015-03-12 Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area Yin, Wenjiao Huang, Changhong Qiu, Feng Liu, Li Wang, Feng Zhou, Jikun Zhang, Yong Bi, Shengli BMC Public Health Research Article BACKGROUND: Illegal commercial plasma and blood donation activities in the late 1980s and early 1990s caused a large number of hepatitis C virus (HCV) infections in rural areas of China. In the present study, we aimed to elucidate the risk factors of HCV RNA positivity and HCV genotype distribution in former blood donors. METHODS: A cross-sectional survey was carried out in a former blood donation village in rural Hebei Province, North China. All residents were invited for a questionnaire interview and testing for HCV antibodies as well as HCV nucleic acids. Questionnaires were administered to collect information about their personal status and commercial blood donation history. Nested PCR was used to amplify HCV nucleic acids in C/E1 region and NS5b region followed by genotyping and phylogenetic analysis. Univariate and multivariate logistic regression were used to analyze the distributions of HCV genotypes in different groups. RESULTS: A total of 512 blood samples were collected. Anti-HCV positive were 148 (28.5%) whereas RNA positive rate was 13.87%. Residents between 50 and 59 years old had the highest RNA positive rate (27/109, 24.77%) (P = 0.0051). Multivariate logistic regression model analysis revealed that plasma donation (OR = 8.666, 95% CI: 1.390-54.025) was the dominant risk factor of HCV infection. Furthermore, HCV subtypes 1b and 2a were found by genotyping and phylogenetic analysis. 36 samples (53.73%) were subtype 1b and 31 samples (46.27%) were subtype 2a. CONCLUSIONS: Unsafe practices during illegal plasma donation led to a high risk of HCV infection. The identification of genotypes 1b and 2a as major HCV genotypes circulating in this region may help to predict the future burden of HCV related diseases and facilitate better medical treatment towards HCV carriers. These results are useful for public healthcare as well as disease control and surveillance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12889-015-1535-6) contains supplementary material, which is available to authorized users. BioMed Central 2015-02-25 /pmc/articles/PMC4355568/ /pubmed/25884321 http://dx.doi.org/10.1186/s12889-015-1535-6 Text en © Yin et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yin, Wenjiao
Huang, Changhong
Qiu, Feng
Liu, Li
Wang, Feng
Zhou, Jikun
Zhang, Yong
Bi, Shengli
Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area
title Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area
title_full Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area
title_fullStr Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area
title_full_unstemmed Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area
title_short Risk factors of hepatitis C virus transmission and genotype distribution in former blood donors from Chinese rural area
title_sort risk factors of hepatitis c virus transmission and genotype distribution in former blood donors from chinese rural area
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355568/
https://www.ncbi.nlm.nih.gov/pubmed/25884321
http://dx.doi.org/10.1186/s12889-015-1535-6
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