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Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum

Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. Although information concerning its genome and transcriptome has recently been reported, relatively little information is available for G. lucidum at the proteomic level. In this study, protein...

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Autores principales: Yu, Guo-Jun, Yin, Ya-Lin, Yu, Wen-Hui, Liu, Wei, Jin, Yan-Xia, Shrestha, Alok, Yang, Qing, Ye, Xiang-Dong, Sun, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355618/
https://www.ncbi.nlm.nih.gov/pubmed/25756518
http://dx.doi.org/10.1371/journal.pone.0119439
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author Yu, Guo-Jun
Yin, Ya-Lin
Yu, Wen-Hui
Liu, Wei
Jin, Yan-Xia
Shrestha, Alok
Yang, Qing
Ye, Xiang-Dong
Sun, Hui
author_facet Yu, Guo-Jun
Yin, Ya-Lin
Yu, Wen-Hui
Liu, Wei
Jin, Yan-Xia
Shrestha, Alok
Yang, Qing
Ye, Xiang-Dong
Sun, Hui
author_sort Yu, Guo-Jun
collection PubMed
description Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. Although information concerning its genome and transcriptome has recently been reported, relatively little information is available for G. lucidum at the proteomic level. In this study, protein fractions from G. lucidum at three developmental stages (16-day mycelia, and fruiting bodies at 60 and 90 days) were prepared and subjected to LC-MS/MS analysis. A search against the G. lucidum genome database identified 803 proteins. Among these proteins, 61 lignocellulose degrading proteins were detected, most of which (49 proteins) were found in the 90-day fruiting bodies. Fourteen TCA-cycle related proteins, 17 peptidases, two argonaute-like proteins, and two immunomodulatory proteins were also detected. A majority (470) of the 803 proteins had GO annotations and were classified into 36 GO terms, with “binding”, “catalytic activity”, and “hydrolase activity” having high percentages. Additionally, 357 out of the 803 proteins were assigned to at least one COG functional category and grouped into 22 COG classifications. Based on the results from the proteomic and sequence alignment analyses, a potentially new immunomodulatory protein (GL18769) was expressed and shown to have high immunomodulatory activity. In this study, proteomic and biochemical analyses of G. lucidum were performed for the first time, revealing that proteins from this fungus can play significant bioactive roles and providing a new foundation for the further functional investigations that this fungus merits.
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spelling pubmed-43556182015-03-17 Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum Yu, Guo-Jun Yin, Ya-Lin Yu, Wen-Hui Liu, Wei Jin, Yan-Xia Shrestha, Alok Yang, Qing Ye, Xiang-Dong Sun, Hui PLoS One Research Article Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. Although information concerning its genome and transcriptome has recently been reported, relatively little information is available for G. lucidum at the proteomic level. In this study, protein fractions from G. lucidum at three developmental stages (16-day mycelia, and fruiting bodies at 60 and 90 days) were prepared and subjected to LC-MS/MS analysis. A search against the G. lucidum genome database identified 803 proteins. Among these proteins, 61 lignocellulose degrading proteins were detected, most of which (49 proteins) were found in the 90-day fruiting bodies. Fourteen TCA-cycle related proteins, 17 peptidases, two argonaute-like proteins, and two immunomodulatory proteins were also detected. A majority (470) of the 803 proteins had GO annotations and were classified into 36 GO terms, with “binding”, “catalytic activity”, and “hydrolase activity” having high percentages. Additionally, 357 out of the 803 proteins were assigned to at least one COG functional category and grouped into 22 COG classifications. Based on the results from the proteomic and sequence alignment analyses, a potentially new immunomodulatory protein (GL18769) was expressed and shown to have high immunomodulatory activity. In this study, proteomic and biochemical analyses of G. lucidum were performed for the first time, revealing that proteins from this fungus can play significant bioactive roles and providing a new foundation for the further functional investigations that this fungus merits. Public Library of Science 2015-03-10 /pmc/articles/PMC4355618/ /pubmed/25756518 http://dx.doi.org/10.1371/journal.pone.0119439 Text en © 2015 Yu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yu, Guo-Jun
Yin, Ya-Lin
Yu, Wen-Hui
Liu, Wei
Jin, Yan-Xia
Shrestha, Alok
Yang, Qing
Ye, Xiang-Dong
Sun, Hui
Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum
title Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum
title_full Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum
title_fullStr Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum
title_full_unstemmed Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum
title_short Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum
title_sort proteome exploration to provide a resource for the investigation of ganoderma lucidum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355618/
https://www.ncbi.nlm.nih.gov/pubmed/25756518
http://dx.doi.org/10.1371/journal.pone.0119439
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