Preparation and Characterization of (125)I Labeled Bovine Serum Albumin

Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to e...

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Autores principales: Ashwitha Rai, K. S., Jyothi, Rasmi, R. R., Sarnaik, Jayula, Kadwad, V. B., Shenoy, K. B., Somashekarappa, H. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355873/
https://www.ncbi.nlm.nih.gov/pubmed/25767326
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author Ashwitha Rai, K. S.
Jyothi,
Rasmi, R. R.
Sarnaik, Jayula
Kadwad, V. B.
Shenoy, K. B.
Somashekarappa, H. M.
author_facet Ashwitha Rai, K. S.
Jyothi,
Rasmi, R. R.
Sarnaik, Jayula
Kadwad, V. B.
Shenoy, K. B.
Somashekarappa, H. M.
author_sort Ashwitha Rai, K. S.
collection PubMed
description Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity.
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spelling pubmed-43558732015-03-12 Preparation and Characterization of (125)I Labeled Bovine Serum Albumin Ashwitha Rai, K. S. Jyothi, Rasmi, R. R. Sarnaik, Jayula Kadwad, V. B. Shenoy, K. B. Somashekarappa, H. M. Indian J Pharm Sci Short Communication Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ((125)I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 µg of chloramine-T along with 20 µg of sodium metabisulphite with 60 seconds incubation at 2° yielded (125)I-BSA with high integrity. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4355873/ /pubmed/25767326 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Ashwitha Rai, K. S.
Jyothi,
Rasmi, R. R.
Sarnaik, Jayula
Kadwad, V. B.
Shenoy, K. B.
Somashekarappa, H. M.
Preparation and Characterization of (125)I Labeled Bovine Serum Albumin
title Preparation and Characterization of (125)I Labeled Bovine Serum Albumin
title_full Preparation and Characterization of (125)I Labeled Bovine Serum Albumin
title_fullStr Preparation and Characterization of (125)I Labeled Bovine Serum Albumin
title_full_unstemmed Preparation and Characterization of (125)I Labeled Bovine Serum Albumin
title_short Preparation and Characterization of (125)I Labeled Bovine Serum Albumin
title_sort preparation and characterization of (125)i labeled bovine serum albumin
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355873/
https://www.ncbi.nlm.nih.gov/pubmed/25767326
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