Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)

PREMISE OF THE STUDY: Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS: Fourteen microsatellite markers were developed. The num...

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Detalles Bibliográficos
Autores principales: Kameoka, Shinichiro, Higashi, Hiroyuki, Setoguchi, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356319/
https://www.ncbi.nlm.nih.gov/pubmed/25798342
http://dx.doi.org/10.3732/apps.1400114
Descripción
Sumario:PREMISE OF THE STUDY: Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS: Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged from 0.043 to 0.855. Eleven markers were successfully amplified in the cultivar ‘Mego’ from Japan. CONCLUSIONS: These microsatellite markers can be used to investigate the genetic diversity, population structure, and gene flow of H. nobilis var. japonica.

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