Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)

PREMISE OF THE STUDY: Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS: Fourteen microsatellite markers were developed. The num...

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Detalles Bibliográficos
Autores principales: Kameoka, Shinichiro, Higashi, Hiroyuki, Setoguchi, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356319/
https://www.ncbi.nlm.nih.gov/pubmed/25798342
http://dx.doi.org/10.3732/apps.1400114
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author Kameoka, Shinichiro
Higashi, Hiroyuki
Setoguchi, Hiroaki
author_facet Kameoka, Shinichiro
Higashi, Hiroyuki
Setoguchi, Hiroaki
author_sort Kameoka, Shinichiro
collection PubMed
description PREMISE OF THE STUDY: Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS: Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged from 0.043 to 0.855. Eleven markers were successfully amplified in the cultivar ‘Mego’ from Japan. CONCLUSIONS: These microsatellite markers can be used to investigate the genetic diversity, population structure, and gene flow of H. nobilis var. japonica.
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spelling pubmed-43563192015-03-20 Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1) Kameoka, Shinichiro Higashi, Hiroyuki Setoguchi, Hiroaki Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. METHODS AND RESULTS: Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged from 0.043 to 0.855. Eleven markers were successfully amplified in the cultivar ‘Mego’ from Japan. CONCLUSIONS: These microsatellite markers can be used to investigate the genetic diversity, population structure, and gene flow of H. nobilis var. japonica. Botanical Society of America 2015-02-18 /pmc/articles/PMC4356319/ /pubmed/25798342 http://dx.doi.org/10.3732/apps.1400114 Text en © 2015 Kameoka et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Kameoka, Shinichiro
Higashi, Hiroyuki
Setoguchi, Hiroaki
Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)
title Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)
title_full Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)
title_fullStr Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)
title_full_unstemmed Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)
title_short Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae)(1)
title_sort development of polymorphic microsatellite loci in the perennial herb hepatica nobilis var. japonica (ranunculaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356319/
https://www.ncbi.nlm.nih.gov/pubmed/25798342
http://dx.doi.org/10.3732/apps.1400114
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