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Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using th...

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Detalles Bibliográficos
Autores principales: Stauber, Mark, Jakoncic, Jean, Berger, Jacob, Karp, Jerome M., Axelbaum, Ariel, Sastow, Dahniel, Buldyrev, Sergey V., Hrnjez, Bruce J., Asherie, Neer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356360/
https://www.ncbi.nlm.nih.gov/pubmed/25760593
http://dx.doi.org/10.1107/S1399004714025061
Descripción
Sumario:Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.