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FEM: feature-enhanced map

A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple meth...

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Detalles Bibliográficos
Autores principales: Afonine, Pavel V., Moriarty, Nigel W., Mustyakimov, Marat, Sobolev, Oleg V., Terwilliger, Thomas C., Turk, Dusan, Urzhumtsev, Alexandre, Adams, Paul D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356370/
https://www.ncbi.nlm.nih.gov/pubmed/25760612
http://dx.doi.org/10.1107/S1399004714028132
Descripción
Sumario:A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple methods. This is followed by restricting the map to regions with convincing density and the application of sharpening. The final map is then created by combining a series of histogram-equalized intermediate maps. In the test cases shown, the maps produced in this way are found to have increased interpretability and decreased model bias compared with the starting 2m F (obs) − D F (model) σ(A)-weighted map.