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FEM: feature-enhanced map
A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple meth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356370/ https://www.ncbi.nlm.nih.gov/pubmed/25760612 http://dx.doi.org/10.1107/S1399004714028132 |
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author | Afonine, Pavel V. Moriarty, Nigel W. Mustyakimov, Marat Sobolev, Oleg V. Terwilliger, Thomas C. Turk, Dusan Urzhumtsev, Alexandre Adams, Paul D. |
author_facet | Afonine, Pavel V. Moriarty, Nigel W. Mustyakimov, Marat Sobolev, Oleg V. Terwilliger, Thomas C. Turk, Dusan Urzhumtsev, Alexandre Adams, Paul D. |
author_sort | Afonine, Pavel V. |
collection | PubMed |
description | A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple methods. This is followed by restricting the map to regions with convincing density and the application of sharpening. The final map is then created by combining a series of histogram-equalized intermediate maps. In the test cases shown, the maps produced in this way are found to have increased interpretability and decreased model bias compared with the starting 2m F (obs) − D F (model) σ(A)-weighted map. |
format | Online Article Text |
id | pubmed-4356370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-43563702015-04-10 FEM: feature-enhanced map Afonine, Pavel V. Moriarty, Nigel W. Mustyakimov, Marat Sobolev, Oleg V. Terwilliger, Thomas C. Turk, Dusan Urzhumtsev, Alexandre Adams, Paul D. Acta Crystallogr D Biol Crystallogr Research Papers A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple methods. This is followed by restricting the map to regions with convincing density and the application of sharpening. The final map is then created by combining a series of histogram-equalized intermediate maps. In the test cases shown, the maps produced in this way are found to have increased interpretability and decreased model bias compared with the starting 2m F (obs) − D F (model) σ(A)-weighted map. International Union of Crystallography 2015-02-26 /pmc/articles/PMC4356370/ /pubmed/25760612 http://dx.doi.org/10.1107/S1399004714028132 Text en © Afonine et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Afonine, Pavel V. Moriarty, Nigel W. Mustyakimov, Marat Sobolev, Oleg V. Terwilliger, Thomas C. Turk, Dusan Urzhumtsev, Alexandre Adams, Paul D. FEM: feature-enhanced map |
title | FEM: feature-enhanced map |
title_full | FEM: feature-enhanced map |
title_fullStr | FEM: feature-enhanced map |
title_full_unstemmed | FEM: feature-enhanced map |
title_short | FEM: feature-enhanced map |
title_sort | fem: feature-enhanced map |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356370/ https://www.ncbi.nlm.nih.gov/pubmed/25760612 http://dx.doi.org/10.1107/S1399004714028132 |
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