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FEM: feature-enhanced map

A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple meth...

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Detalles Bibliográficos
Autores principales: Afonine, Pavel V., Moriarty, Nigel W., Mustyakimov, Marat, Sobolev, Oleg V., Terwilliger, Thomas C., Turk, Dusan, Urzhumtsev, Alexandre, Adams, Paul D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356370/
https://www.ncbi.nlm.nih.gov/pubmed/25760612
http://dx.doi.org/10.1107/S1399004714028132
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author Afonine, Pavel V.
Moriarty, Nigel W.
Mustyakimov, Marat
Sobolev, Oleg V.
Terwilliger, Thomas C.
Turk, Dusan
Urzhumtsev, Alexandre
Adams, Paul D.
author_facet Afonine, Pavel V.
Moriarty, Nigel W.
Mustyakimov, Marat
Sobolev, Oleg V.
Terwilliger, Thomas C.
Turk, Dusan
Urzhumtsev, Alexandre
Adams, Paul D.
author_sort Afonine, Pavel V.
collection PubMed
description A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple methods. This is followed by restricting the map to regions with convincing density and the application of sharpening. The final map is then created by combining a series of histogram-equalized intermediate maps. In the test cases shown, the maps produced in this way are found to have increased interpretability and decreased model bias compared with the starting 2m F (obs) − D F (model) σ(A)-weighted map.
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spelling pubmed-43563702015-04-10 FEM: feature-enhanced map Afonine, Pavel V. Moriarty, Nigel W. Mustyakimov, Marat Sobolev, Oleg V. Terwilliger, Thomas C. Turk, Dusan Urzhumtsev, Alexandre Adams, Paul D. Acta Crystallogr D Biol Crystallogr Research Papers A method is presented that modifies a 2m F (obs) − D F (model) σ(A)-weighted map such that the resulting map can strengthen a weak signal, if present, and can reduce model bias and noise. The method consists of first randomizing the starting map and filling in missing reflections using multiple methods. This is followed by restricting the map to regions with convincing density and the application of sharpening. The final map is then created by combining a series of histogram-equalized intermediate maps. In the test cases shown, the maps produced in this way are found to have increased interpretability and decreased model bias compared with the starting 2m F (obs) − D F (model) σ(A)-weighted map. International Union of Crystallography 2015-02-26 /pmc/articles/PMC4356370/ /pubmed/25760612 http://dx.doi.org/10.1107/S1399004714028132 Text en © Afonine et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Afonine, Pavel V.
Moriarty, Nigel W.
Mustyakimov, Marat
Sobolev, Oleg V.
Terwilliger, Thomas C.
Turk, Dusan
Urzhumtsev, Alexandre
Adams, Paul D.
FEM: feature-enhanced map
title FEM: feature-enhanced map
title_full FEM: feature-enhanced map
title_fullStr FEM: feature-enhanced map
title_full_unstemmed FEM: feature-enhanced map
title_short FEM: feature-enhanced map
title_sort fem: feature-enhanced map
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356370/
https://www.ncbi.nlm.nih.gov/pubmed/25760612
http://dx.doi.org/10.1107/S1399004714028132
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