The structure of the hexameric atrazine chlorohydrolase AtzA

Atrazine chlorohydrolase (AtzA) was discovered and purified in the early 1990s from soil that had been exposed to the widely used herbicide atrazine. It was subsequently found that this enzyme catalyzes the first and necessary step in the breakdown of atrazine by the soil organism Pseudomonas sp. st...

Descripción completa

Detalles Bibliográficos
Autores principales: Peat, T. S., Newman, J., Balotra, S., Lucent, D., Warden, A. C., Scott, C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356373/
https://www.ncbi.nlm.nih.gov/pubmed/25760618
http://dx.doi.org/10.1107/S1399004715000619
_version_ 1782360988835643392
author Peat, T. S.
Newman, J.
Balotra, S.
Lucent, D.
Warden, A. C.
Scott, C.
author_facet Peat, T. S.
Newman, J.
Balotra, S.
Lucent, D.
Warden, A. C.
Scott, C.
author_sort Peat, T. S.
collection PubMed
description Atrazine chlorohydrolase (AtzA) was discovered and purified in the early 1990s from soil that had been exposed to the widely used herbicide atrazine. It was subsequently found that this enzyme catalyzes the first and necessary step in the breakdown of atrazine by the soil organism Pseudomonas sp. strain ADP. Although it has taken 20 years, a crystal structure of the full hexameric form of AtzA has now been obtained. AtzA is less well adapted to its physiological role (i.e. atrazine dechlorination) than the alternative metal-dependent atrazine chlorohydrolase (TrzN), with a substrate-binding pocket that is under considerable strain and for which the substrate is a poor fit.
format Online
Article
Text
id pubmed-4356373
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-43563732015-04-10 The structure of the hexameric atrazine chlorohydrolase AtzA Peat, T. S. Newman, J. Balotra, S. Lucent, D. Warden, A. C. Scott, C. Acta Crystallogr D Biol Crystallogr Research Papers Atrazine chlorohydrolase (AtzA) was discovered and purified in the early 1990s from soil that had been exposed to the widely used herbicide atrazine. It was subsequently found that this enzyme catalyzes the first and necessary step in the breakdown of atrazine by the soil organism Pseudomonas sp. strain ADP. Although it has taken 20 years, a crystal structure of the full hexameric form of AtzA has now been obtained. AtzA is less well adapted to its physiological role (i.e. atrazine dechlorination) than the alternative metal-dependent atrazine chlorohydrolase (TrzN), with a substrate-binding pocket that is under considerable strain and for which the substrate is a poor fit. International Union of Crystallography 2015-02-26 /pmc/articles/PMC4356373/ /pubmed/25760618 http://dx.doi.org/10.1107/S1399004715000619 Text en © Peat et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Peat, T. S.
Newman, J.
Balotra, S.
Lucent, D.
Warden, A. C.
Scott, C.
The structure of the hexameric atrazine chlorohydrolase AtzA
title The structure of the hexameric atrazine chlorohydrolase AtzA
title_full The structure of the hexameric atrazine chlorohydrolase AtzA
title_fullStr The structure of the hexameric atrazine chlorohydrolase AtzA
title_full_unstemmed The structure of the hexameric atrazine chlorohydrolase AtzA
title_short The structure of the hexameric atrazine chlorohydrolase AtzA
title_sort structure of the hexameric atrazine chlorohydrolase atza
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356373/
https://www.ncbi.nlm.nih.gov/pubmed/25760618
http://dx.doi.org/10.1107/S1399004715000619
work_keys_str_mv AT peatts thestructureofthehexamericatrazinechlorohydrolaseatza
AT newmanj thestructureofthehexamericatrazinechlorohydrolaseatza
AT balotras thestructureofthehexamericatrazinechlorohydrolaseatza
AT lucentd thestructureofthehexamericatrazinechlorohydrolaseatza
AT wardenac thestructureofthehexamericatrazinechlorohydrolaseatza
AT scottc thestructureofthehexamericatrazinechlorohydrolaseatza
AT peatts structureofthehexamericatrazinechlorohydrolaseatza
AT newmanj structureofthehexamericatrazinechlorohydrolaseatza
AT balotras structureofthehexamericatrazinechlorohydrolaseatza
AT lucentd structureofthehexamericatrazinechlorohydrolaseatza
AT wardenac structureofthehexamericatrazinechlorohydrolaseatza
AT scottc structureofthehexamericatrazinechlorohydrolaseatza

Ejemplares similares