Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR

Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected...

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Detalles Bibliográficos
Autores principales: Inoue, Hiroaki, Fujimura, Reiko, Agata, Kunio, Ohta, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356457/
https://www.ncbi.nlm.nih.gov/pubmed/25736979
http://dx.doi.org/10.1264/jsme2.ME14115
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author Inoue, Hiroaki
Fujimura, Reiko
Agata, Kunio
Ohta, Hiroyuki
author_facet Inoue, Hiroaki
Fujimura, Reiko
Agata, Kunio
Ohta, Hiroyuki
author_sort Inoue, Hiroaki
collection PubMed
description Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments.
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spelling pubmed-43564572015-03-12 Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR Inoue, Hiroaki Fujimura, Reiko Agata, Kunio Ohta, Hiroyuki Microbes Environ Short Communication Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments. The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2015-03 2015-01-16 /pmc/articles/PMC4356457/ /pubmed/25736979 http://dx.doi.org/10.1264/jsme2.ME14115 Text en Copyright 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Inoue, Hiroaki
Fujimura, Reiko
Agata, Kunio
Ohta, Hiroyuki
Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
title Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
title_full Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
title_fullStr Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
title_full_unstemmed Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
title_short Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR
title_sort molecular characterization of viable legionella spp. in cooling tower water samples by combined use of ethidium monoazide and pcr
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356457/
https://www.ncbi.nlm.nih.gov/pubmed/25736979
http://dx.doi.org/10.1264/jsme2.ME14115
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