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Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions

Rice shoot-associated bacterial communities at the panicle initiation stage were characterized and their responses to elevated surface water-soil temperature (ET), low nitrogen (LN), and free-air CO(2) enrichment (FACE) were assessed by clone library analyses of the 16S rRNA gene. Principal coordina...

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Autores principales: Ikeda, Seishi, Tokida, Takeshi, Nakamura, Hirofumi, Sakai, Hidemitsu, Usui, Yasuhiro, Okubo, Takashi, Tago, Kanako, Hayashi, Kentaro, Sekiyama, Yasuyo, Ono, Hiroshi, Tomita, Satoru, Hayatsu, Masahito, Hasegawa, Toshihiro, Minamisawa, Kiwamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356464/
https://www.ncbi.nlm.nih.gov/pubmed/25740174
http://dx.doi.org/10.1264/jsme2.ME14117
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author Ikeda, Seishi
Tokida, Takeshi
Nakamura, Hirofumi
Sakai, Hidemitsu
Usui, Yasuhiro
Okubo, Takashi
Tago, Kanako
Hayashi, Kentaro
Sekiyama, Yasuyo
Ono, Hiroshi
Tomita, Satoru
Hayatsu, Masahito
Hasegawa, Toshihiro
Minamisawa, Kiwamu
author_facet Ikeda, Seishi
Tokida, Takeshi
Nakamura, Hirofumi
Sakai, Hidemitsu
Usui, Yasuhiro
Okubo, Takashi
Tago, Kanako
Hayashi, Kentaro
Sekiyama, Yasuyo
Ono, Hiroshi
Tomita, Satoru
Hayatsu, Masahito
Hasegawa, Toshihiro
Minamisawa, Kiwamu
author_sort Ikeda, Seishi
collection PubMed
description Rice shoot-associated bacterial communities at the panicle initiation stage were characterized and their responses to elevated surface water-soil temperature (ET), low nitrogen (LN), and free-air CO(2) enrichment (FACE) were assessed by clone library analyses of the 16S rRNA gene. Principal coordinate analyses combining all sequence data for leaf blade- and leaf sheath-associated bacteria revealed that each bacterial community had a distinct structure, as supported by PC1 (61.5%), that was mainly attributed to the high abundance of Planctomycetes in leaf sheaths. Our results also indicated that the community structures of leaf blade-associated bacteria were more sensitive than those of leaf sheath-associated bacteria to the environmental factors examined. Among these environmental factors, LN strongly affected the community structures of leaf blade-associated bacteria by increasing the relative abundance of Bacilli. The most significant effect of FACE was also observed on leaf blade-associated bacteria under the LN condition, which was explained by decreases and increases in Agrobacterium and Pantoea, respectively. The community structures of leaf blade-associated bacteria under the combination of FACE and ET were more similar to those of the control than to those under ET or FACE. Thus, the combined effects of environmental factors need to be considered in order to realistically assess the effects of environmental changes on microbial community structures.
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spelling pubmed-43564642015-03-12 Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions Ikeda, Seishi Tokida, Takeshi Nakamura, Hirofumi Sakai, Hidemitsu Usui, Yasuhiro Okubo, Takashi Tago, Kanako Hayashi, Kentaro Sekiyama, Yasuyo Ono, Hiroshi Tomita, Satoru Hayatsu, Masahito Hasegawa, Toshihiro Minamisawa, Kiwamu Microbes Environ Articles Rice shoot-associated bacterial communities at the panicle initiation stage were characterized and their responses to elevated surface water-soil temperature (ET), low nitrogen (LN), and free-air CO(2) enrichment (FACE) were assessed by clone library analyses of the 16S rRNA gene. Principal coordinate analyses combining all sequence data for leaf blade- and leaf sheath-associated bacteria revealed that each bacterial community had a distinct structure, as supported by PC1 (61.5%), that was mainly attributed to the high abundance of Planctomycetes in leaf sheaths. Our results also indicated that the community structures of leaf blade-associated bacteria were more sensitive than those of leaf sheath-associated bacteria to the environmental factors examined. Among these environmental factors, LN strongly affected the community structures of leaf blade-associated bacteria by increasing the relative abundance of Bacilli. The most significant effect of FACE was also observed on leaf blade-associated bacteria under the LN condition, which was explained by decreases and increases in Agrobacterium and Pantoea, respectively. The community structures of leaf blade-associated bacteria under the combination of FACE and ET were more similar to those of the control than to those under ET or FACE. Thus, the combined effects of environmental factors need to be considered in order to realistically assess the effects of environmental changes on microbial community structures. The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2015-03 2015-02-04 /pmc/articles/PMC4356464/ /pubmed/25740174 http://dx.doi.org/10.1264/jsme2.ME14117 Text en Copyright 2015 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Ikeda, Seishi
Tokida, Takeshi
Nakamura, Hirofumi
Sakai, Hidemitsu
Usui, Yasuhiro
Okubo, Takashi
Tago, Kanako
Hayashi, Kentaro
Sekiyama, Yasuyo
Ono, Hiroshi
Tomita, Satoru
Hayatsu, Masahito
Hasegawa, Toshihiro
Minamisawa, Kiwamu
Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions
title Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions
title_full Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions
title_fullStr Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions
title_full_unstemmed Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions
title_short Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO2, Temperature, and Nitrogen Levels under Field Conditions
title_sort characterization of leaf blade- and leaf sheath-associated bacterial communities and assessment of their responses to environmental changes in co2, temperature, and nitrogen levels under field conditions
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356464/
https://www.ncbi.nlm.nih.gov/pubmed/25740174
http://dx.doi.org/10.1264/jsme2.ME14117
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