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A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer

Epidermal growth factor receptor (EGFR) gene mutation testing is essential for choosing appropriate treatment options in patients with advanced non-small cell lung cancer (NSCLC). However, a time delay occurs between histological diagnosis and molecular diagnosis in clinical situations. To minimize...

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Autores principales: SAKAMOTO, TOMOHIRO, KODANI, MASAHIRO, TAKATA, MIYAKO, CHIKUMI, HIROKI, NAKAMOTO, MASAKI, NISHII-ITO, SHIZUKA, UEDA, YASUTO, IZUMI, HIROKI, MAKINO, HARUHIKO, TOUGE, HIROKAZU, TAKEDA, KENICHI, YAMASAKI, AKIRA, YANAI, MASAAKI, TANAKA, NATSUMI, IGISHI, TADASHI, SHIMIZU, EIJI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356493/
https://www.ncbi.nlm.nih.gov/pubmed/25651992
http://dx.doi.org/10.3892/ijo.2015.2875
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author SAKAMOTO, TOMOHIRO
KODANI, MASAHIRO
TAKATA, MIYAKO
CHIKUMI, HIROKI
NAKAMOTO, MASAKI
NISHII-ITO, SHIZUKA
UEDA, YASUTO
IZUMI, HIROKI
MAKINO, HARUHIKO
TOUGE, HIROKAZU
TAKEDA, KENICHI
YAMASAKI, AKIRA
YANAI, MASAAKI
TANAKA, NATSUMI
IGISHI, TADASHI
SHIMIZU, EIJI
author_facet SAKAMOTO, TOMOHIRO
KODANI, MASAHIRO
TAKATA, MIYAKO
CHIKUMI, HIROKI
NAKAMOTO, MASAKI
NISHII-ITO, SHIZUKA
UEDA, YASUTO
IZUMI, HIROKI
MAKINO, HARUHIKO
TOUGE, HIROKAZU
TAKEDA, KENICHI
YAMASAKI, AKIRA
YANAI, MASAAKI
TANAKA, NATSUMI
IGISHI, TADASHI
SHIMIZU, EIJI
author_sort SAKAMOTO, TOMOHIRO
collection PubMed
description Epidermal growth factor receptor (EGFR) gene mutation testing is essential for choosing appropriate treatment options in patients with advanced non-small cell lung cancer (NSCLC). However, a time delay occurs between histological diagnosis and molecular diagnosis in clinical situations. To minimize this delay, we developed a novel point-of-care test for EGFR mutations, based on a high-speed real-time polymerase chain reaction (PCR) system designated here as ultrarapid PCR combined with highly accurate bronchoscopic sampling. We investigated whether our system for detecting EGFR mutations was valid by comparing test results with those obtained using a commercialized EGFR mutation test. We obtained small amounts of bronchial lavage fluids after transbronchial biopsies (TBBs) were performed on enrolled patients (n=168) who underwent endobronchial ultrasonography using a guide sheath (EBUS-GS). EGFR mutation analysis was performed by ultrarapid PCR immediately after EBUS-GS-TBBs were obtained (on the same day). After pathological diagnoses of NSCLC, EGFR mutation status in formalin-fixed, paraffin- embedded samples was confirmed by the PCR-invader method, and the concordance rates between the PCR methods were compared. The total diagnostic yield of EBUS-GS-TBB was 91.0%. The positive concordance rates for detecting 19del and L858R with the ultrarapid PCR and PCR-invader methods were both 100%. Negative concordance rates were 97.2 and 98.1%, respectively. We also demonstrated a dramatic effect of early erlotinib administration, based on ultrarapid PCR results, for a 52-year-old woman suffering from respiratory failure due to severe intrapulmonary metastases with poor performance status. In conclusion, ultrarapid PCR combined with EBUS-GS-TBB enabled rapid and reliable point-of-care testing for EGFR mutations.
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spelling pubmed-43564932015-03-18 A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer SAKAMOTO, TOMOHIRO KODANI, MASAHIRO TAKATA, MIYAKO CHIKUMI, HIROKI NAKAMOTO, MASAKI NISHII-ITO, SHIZUKA UEDA, YASUTO IZUMI, HIROKI MAKINO, HARUHIKO TOUGE, HIROKAZU TAKEDA, KENICHI YAMASAKI, AKIRA YANAI, MASAAKI TANAKA, NATSUMI IGISHI, TADASHI SHIMIZU, EIJI Int J Oncol Articles Epidermal growth factor receptor (EGFR) gene mutation testing is essential for choosing appropriate treatment options in patients with advanced non-small cell lung cancer (NSCLC). However, a time delay occurs between histological diagnosis and molecular diagnosis in clinical situations. To minimize this delay, we developed a novel point-of-care test for EGFR mutations, based on a high-speed real-time polymerase chain reaction (PCR) system designated here as ultrarapid PCR combined with highly accurate bronchoscopic sampling. We investigated whether our system for detecting EGFR mutations was valid by comparing test results with those obtained using a commercialized EGFR mutation test. We obtained small amounts of bronchial lavage fluids after transbronchial biopsies (TBBs) were performed on enrolled patients (n=168) who underwent endobronchial ultrasonography using a guide sheath (EBUS-GS). EGFR mutation analysis was performed by ultrarapid PCR immediately after EBUS-GS-TBBs were obtained (on the same day). After pathological diagnoses of NSCLC, EGFR mutation status in formalin-fixed, paraffin- embedded samples was confirmed by the PCR-invader method, and the concordance rates between the PCR methods were compared. The total diagnostic yield of EBUS-GS-TBB was 91.0%. The positive concordance rates for detecting 19del and L858R with the ultrarapid PCR and PCR-invader methods were both 100%. Negative concordance rates were 97.2 and 98.1%, respectively. We also demonstrated a dramatic effect of early erlotinib administration, based on ultrarapid PCR results, for a 52-year-old woman suffering from respiratory failure due to severe intrapulmonary metastases with poor performance status. In conclusion, ultrarapid PCR combined with EBUS-GS-TBB enabled rapid and reliable point-of-care testing for EGFR mutations. D.A. Spandidos 2015-02-04 /pmc/articles/PMC4356493/ /pubmed/25651992 http://dx.doi.org/10.3892/ijo.2015.2875 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
SAKAMOTO, TOMOHIRO
KODANI, MASAHIRO
TAKATA, MIYAKO
CHIKUMI, HIROKI
NAKAMOTO, MASAKI
NISHII-ITO, SHIZUKA
UEDA, YASUTO
IZUMI, HIROKI
MAKINO, HARUHIKO
TOUGE, HIROKAZU
TAKEDA, KENICHI
YAMASAKI, AKIRA
YANAI, MASAAKI
TANAKA, NATSUMI
IGISHI, TADASHI
SHIMIZU, EIJI
A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
title A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
title_full A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
title_fullStr A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
title_full_unstemmed A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
title_short A novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
title_sort novel point-of-care system for high-speed real-time polymerase chain reaction testing for epidermal growth factor receptor mutations in bronchial lavage fluids after transbronchial biopsy in patients with non-small cell lung cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356493/
https://www.ncbi.nlm.nih.gov/pubmed/25651992
http://dx.doi.org/10.3892/ijo.2015.2875
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