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Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging
Signal transducers and activators of transcription (STATs) can transduce cytokine signals and regulate gene expression. The cellular localization and nuclear trafficking of STAT1, a representative of the STAT family with multiple transcriptional functions, is tightly related with transcription proce...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356954/ https://www.ncbi.nlm.nih.gov/pubmed/25762114 http://dx.doi.org/10.1038/srep09045 |
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author | Gao, Jing Wang, Feng Liu, Yanhou Cai, Mingjun Xu, Haijiao Jiang, Junguang Wang, Hongda |
author_facet | Gao, Jing Wang, Feng Liu, Yanhou Cai, Mingjun Xu, Haijiao Jiang, Junguang Wang, Hongda |
author_sort | Gao, Jing |
collection | PubMed |
description | Signal transducers and activators of transcription (STATs) can transduce cytokine signals and regulate gene expression. The cellular localization and nuclear trafficking of STAT1, a representative of the STAT family with multiple transcriptional functions, is tightly related with transcription process, which usually happens in the interphase of the cell cycle. However, these priority questions regarding STAT1 distribution and localization at the different cell-cycle stages remain unclear. By using direct stochastic optical reconstruction microscopy (dSTORM), we found that the nuclear expression level of STAT1 increased gradually as the cell cycle carried out, especially after EGF stimulation. Furthermore, STAT1 formed clusters in the whole cell during the cell cycle, with the size and the number of clusters also increasing significantly from G1 to G2 phase, suggesting that transcription and other cell-cycle related activities can promote STAT1 to form more and larger clusters for fast response to signals. Our work reveals that the cellular localization and clustering distribution of STAT1 are associated with the cell cycle, and further provides an insight into the mechanism of cell-cycle regulated STAT1 signal transduction. |
format | Online Article Text |
id | pubmed-4356954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43569542015-03-17 Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging Gao, Jing Wang, Feng Liu, Yanhou Cai, Mingjun Xu, Haijiao Jiang, Junguang Wang, Hongda Sci Rep Article Signal transducers and activators of transcription (STATs) can transduce cytokine signals and regulate gene expression. The cellular localization and nuclear trafficking of STAT1, a representative of the STAT family with multiple transcriptional functions, is tightly related with transcription process, which usually happens in the interphase of the cell cycle. However, these priority questions regarding STAT1 distribution and localization at the different cell-cycle stages remain unclear. By using direct stochastic optical reconstruction microscopy (dSTORM), we found that the nuclear expression level of STAT1 increased gradually as the cell cycle carried out, especially after EGF stimulation. Furthermore, STAT1 formed clusters in the whole cell during the cell cycle, with the size and the number of clusters also increasing significantly from G1 to G2 phase, suggesting that transcription and other cell-cycle related activities can promote STAT1 to form more and larger clusters for fast response to signals. Our work reveals that the cellular localization and clustering distribution of STAT1 are associated with the cell cycle, and further provides an insight into the mechanism of cell-cycle regulated STAT1 signal transduction. Nature Publishing Group 2015-03-12 /pmc/articles/PMC4356954/ /pubmed/25762114 http://dx.doi.org/10.1038/srep09045 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Gao, Jing Wang, Feng Liu, Yanhou Cai, Mingjun Xu, Haijiao Jiang, Junguang Wang, Hongda Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging |
title | Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging |
title_full | Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging |
title_fullStr | Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging |
title_full_unstemmed | Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging |
title_short | Revealing the cellular localization of STAT1 during the cell cycle by super-resolution imaging |
title_sort | revealing the cellular localization of stat1 during the cell cycle by super-resolution imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356954/ https://www.ncbi.nlm.nih.gov/pubmed/25762114 http://dx.doi.org/10.1038/srep09045 |
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