Four-dimensional in vivo X-ray microscopy with projection-guided gating

Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microsec...

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Autores principales: Mokso, Rajmund, Schwyn, Daniel A., Walker, Simon M., Doube, Michael, Wicklein, Martina, Müller, Tonya, Stampanoni, Marco, Taylor, Graham K., Krapp, Holger G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356984/
https://www.ncbi.nlm.nih.gov/pubmed/25762080
http://dx.doi.org/10.1038/srep08727
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author Mokso, Rajmund
Schwyn, Daniel A.
Walker, Simon M.
Doube, Michael
Wicklein, Martina
Müller, Tonya
Stampanoni, Marco
Taylor, Graham K.
Krapp, Holger G.
author_facet Mokso, Rajmund
Schwyn, Daniel A.
Walker, Simon M.
Doube, Michael
Wicklein, Martina
Müller, Tonya
Stampanoni, Marco
Taylor, Graham K.
Krapp, Holger G.
author_sort Mokso, Rajmund
collection PubMed
description Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.
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spelling pubmed-43569842015-03-17 Four-dimensional in vivo X-ray microscopy with projection-guided gating Mokso, Rajmund Schwyn, Daniel A. Walker, Simon M. Doube, Michael Wicklein, Martina Müller, Tonya Stampanoni, Marco Taylor, Graham K. Krapp, Holger G. Sci Rep Article Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor. Nature Publishing Group 2015-03-12 /pmc/articles/PMC4356984/ /pubmed/25762080 http://dx.doi.org/10.1038/srep08727 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mokso, Rajmund
Schwyn, Daniel A.
Walker, Simon M.
Doube, Michael
Wicklein, Martina
Müller, Tonya
Stampanoni, Marco
Taylor, Graham K.
Krapp, Holger G.
Four-dimensional in vivo X-ray microscopy with projection-guided gating
title Four-dimensional in vivo X-ray microscopy with projection-guided gating
title_full Four-dimensional in vivo X-ray microscopy with projection-guided gating
title_fullStr Four-dimensional in vivo X-ray microscopy with projection-guided gating
title_full_unstemmed Four-dimensional in vivo X-ray microscopy with projection-guided gating
title_short Four-dimensional in vivo X-ray microscopy with projection-guided gating
title_sort four-dimensional in vivo x-ray microscopy with projection-guided gating
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356984/
https://www.ncbi.nlm.nih.gov/pubmed/25762080
http://dx.doi.org/10.1038/srep08727
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