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Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia

This study investigates the development of the spatiotemporal pattern of action potential alternans during acute regional ischemia. Experiments were carried out in isolated Langendorff-perfused rabbit heart using a combination of optical mapping and microelectrode recordings. The alternans pattern s...

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Autores principales: Martišienė, Irma, Jurevičius, Jonas, Vosyliūtė, Rūta, Navalinskas, Antanas, Treinys, Rimantas, Mačianskienė, Regina, Benetis, Rimantas, Matiukas, Arvydas, Pertsov, Arkady M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357036/
https://www.ncbi.nlm.nih.gov/pubmed/25815342
http://dx.doi.org/10.1155/2015/951704
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author Martišienė, Irma
Jurevičius, Jonas
Vosyliūtė, Rūta
Navalinskas, Antanas
Treinys, Rimantas
Mačianskienė, Regina
Benetis, Rimantas
Matiukas, Arvydas
Pertsov, Arkady M.
author_facet Martišienė, Irma
Jurevičius, Jonas
Vosyliūtė, Rūta
Navalinskas, Antanas
Treinys, Rimantas
Mačianskienė, Regina
Benetis, Rimantas
Matiukas, Arvydas
Pertsov, Arkady M.
author_sort Martišienė, Irma
collection PubMed
description This study investigates the development of the spatiotemporal pattern of action potential alternans during acute regional ischemia. Experiments were carried out in isolated Langendorff-perfused rabbit heart using a combination of optical mapping and microelectrode recordings. The alternans pattern significantly changed over time and had a biphasic character reaching maximum at 6–9 min after occlusion. Phase I (3–11 minutes of ischemia) is characterized by rapid increase in the alternans magnitude and expansion of the alternans territory. Phase I is followed by gradual decline of alternans (Phase II) in both magnitude and territory. During both phases we observed significant beat-to-beat variations of the optical action potential amplitude (OAPA) alternans. Simultaneous microelectrode recordings from subepicardial and subendocardial layers showed that OAPA alternans coincided with intramural 2 : 1 conduction blocks. Our findings are consistent with the modeling studies predicting that during acute regional ischemia alternans can be driven by 2 : 1 conduction blocks in the ischemic region.
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spelling pubmed-43570362015-03-26 Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia Martišienė, Irma Jurevičius, Jonas Vosyliūtė, Rūta Navalinskas, Antanas Treinys, Rimantas Mačianskienė, Regina Benetis, Rimantas Matiukas, Arvydas Pertsov, Arkady M. Biomed Res Int Research Article This study investigates the development of the spatiotemporal pattern of action potential alternans during acute regional ischemia. Experiments were carried out in isolated Langendorff-perfused rabbit heart using a combination of optical mapping and microelectrode recordings. The alternans pattern significantly changed over time and had a biphasic character reaching maximum at 6–9 min after occlusion. Phase I (3–11 minutes of ischemia) is characterized by rapid increase in the alternans magnitude and expansion of the alternans territory. Phase I is followed by gradual decline of alternans (Phase II) in both magnitude and territory. During both phases we observed significant beat-to-beat variations of the optical action potential amplitude (OAPA) alternans. Simultaneous microelectrode recordings from subepicardial and subendocardial layers showed that OAPA alternans coincided with intramural 2 : 1 conduction blocks. Our findings are consistent with the modeling studies predicting that during acute regional ischemia alternans can be driven by 2 : 1 conduction blocks in the ischemic region. Hindawi Publishing Corporation 2015 2015-02-26 /pmc/articles/PMC4357036/ /pubmed/25815342 http://dx.doi.org/10.1155/2015/951704 Text en Copyright © 2015 Irma Martišienė et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Martišienė, Irma
Jurevičius, Jonas
Vosyliūtė, Rūta
Navalinskas, Antanas
Treinys, Rimantas
Mačianskienė, Regina
Benetis, Rimantas
Matiukas, Arvydas
Pertsov, Arkady M.
Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia
title Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia
title_full Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia
title_fullStr Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia
title_full_unstemmed Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia
title_short Evolution of Action Potential Alternans in Rabbit Heart during Acute Regional Ischemia
title_sort evolution of action potential alternans in rabbit heart during acute regional ischemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357036/
https://www.ncbi.nlm.nih.gov/pubmed/25815342
http://dx.doi.org/10.1155/2015/951704
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