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Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes

The retromer complex facilitates the sorting of integral membrane proteins from the endosome to the late Golgi. In mammalian cells, the efficient recruitment of retromer to endosomes requires the lipid phosphatidylinositol 3-phosphate (PI3P) as well as Rab5 and Rab7 GTPases. However, in yeast, the r...

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Autores principales: Bean, Bjorn D. M., Davey, Michael, Snider, Jamie, Jessulat, Matthew, Deineko, Viktor, Tinney, Matthew, Stagljar, Igor, Babu, Mohan, Conibear, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357511/
https://www.ncbi.nlm.nih.gov/pubmed/25609093
http://dx.doi.org/10.1091/mbc.E14-08-1281
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author Bean, Bjorn D. M.
Davey, Michael
Snider, Jamie
Jessulat, Matthew
Deineko, Viktor
Tinney, Matthew
Stagljar, Igor
Babu, Mohan
Conibear, Elizabeth
author_facet Bean, Bjorn D. M.
Davey, Michael
Snider, Jamie
Jessulat, Matthew
Deineko, Viktor
Tinney, Matthew
Stagljar, Igor
Babu, Mohan
Conibear, Elizabeth
author_sort Bean, Bjorn D. M.
collection PubMed
description The retromer complex facilitates the sorting of integral membrane proteins from the endosome to the late Golgi. In mammalian cells, the efficient recruitment of retromer to endosomes requires the lipid phosphatidylinositol 3-phosphate (PI3P) as well as Rab5 and Rab7 GTPases. However, in yeast, the role of Rabs in recruiting retromer to endosomes is less clear. We identified novel physical interactions between retromer and the Saccharomyces cerevisiae VPS9-domain Rab5-family guanine nucleotide exchange factors (GEFs) Muk1 and Vps9. Furthermore, we identified a new yeast VPS9 domain-containing protein, VARP-like 1 (Vrl1), which is related to the human VARP protein. All three VPS9 domain–containing proteins show localization to endosomes, and the presence of any one of them is necessary for the endosomal recruitment of retromer. We find that expression of an active VPS9-domain protein is required for correct localization of the phosphatidylinositol 3-kinase Vps34 and the production of endosomal PI3P. These results suggest that VPS9 GEFs promote retromer recruitment by establishing PI3P-enriched domains at the endosomal membrane. The interaction of retromer with distinct VPS9 GEFs could thus link GEF-dependent regulatory inputs to the temporal or spatial coordination of retromer assembly or function.
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spelling pubmed-43575112015-05-30 Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes Bean, Bjorn D. M. Davey, Michael Snider, Jamie Jessulat, Matthew Deineko, Viktor Tinney, Matthew Stagljar, Igor Babu, Mohan Conibear, Elizabeth Mol Biol Cell Articles The retromer complex facilitates the sorting of integral membrane proteins from the endosome to the late Golgi. In mammalian cells, the efficient recruitment of retromer to endosomes requires the lipid phosphatidylinositol 3-phosphate (PI3P) as well as Rab5 and Rab7 GTPases. However, in yeast, the role of Rabs in recruiting retromer to endosomes is less clear. We identified novel physical interactions between retromer and the Saccharomyces cerevisiae VPS9-domain Rab5-family guanine nucleotide exchange factors (GEFs) Muk1 and Vps9. Furthermore, we identified a new yeast VPS9 domain-containing protein, VARP-like 1 (Vrl1), which is related to the human VARP protein. All three VPS9 domain–containing proteins show localization to endosomes, and the presence of any one of them is necessary for the endosomal recruitment of retromer. We find that expression of an active VPS9-domain protein is required for correct localization of the phosphatidylinositol 3-kinase Vps34 and the production of endosomal PI3P. These results suggest that VPS9 GEFs promote retromer recruitment by establishing PI3P-enriched domains at the endosomal membrane. The interaction of retromer with distinct VPS9 GEFs could thus link GEF-dependent regulatory inputs to the temporal or spatial coordination of retromer assembly or function. The American Society for Cell Biology 2015-03-15 /pmc/articles/PMC4357511/ /pubmed/25609093 http://dx.doi.org/10.1091/mbc.E14-08-1281 Text en © 2015 Bean et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Bean, Bjorn D. M.
Davey, Michael
Snider, Jamie
Jessulat, Matthew
Deineko, Viktor
Tinney, Matthew
Stagljar, Igor
Babu, Mohan
Conibear, Elizabeth
Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
title Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
title_full Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
title_fullStr Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
title_full_unstemmed Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
title_short Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
title_sort rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357511/
https://www.ncbi.nlm.nih.gov/pubmed/25609093
http://dx.doi.org/10.1091/mbc.E14-08-1281
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