The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology

DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and w...

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Detalles Bibliográficos
Autores principales: Chen, Fei, Dong, Mengxing, Ge, Meng, Zhu, Lingxiang, Ren, Lufeng, Liu, Guocheng, Mu, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357665/
https://www.ncbi.nlm.nih.gov/pubmed/23414612
http://dx.doi.org/10.1016/j.gpb.2013.01.003
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author Chen, Fei
Dong, Mengxing
Ge, Meng
Zhu, Lingxiang
Ren, Lufeng
Liu, Guocheng
Mu, Rong
author_facet Chen, Fei
Dong, Mengxing
Ge, Meng
Zhu, Lingxiang
Ren, Lufeng
Liu, Guocheng
Mu, Rong
author_sort Chen, Fei
collection PubMed
description DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the “REAP” method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application.
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spelling pubmed-43576652015-05-06 The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology Chen, Fei Dong, Mengxing Ge, Meng Zhu, Lingxiang Ren, Lufeng Liu, Guocheng Mu, Rong Genomics Proteomics Bioinformatics Review DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the “REAP” method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application. Elsevier 2013-02 2013-01-23 /pmc/articles/PMC4357665/ /pubmed/23414612 http://dx.doi.org/10.1016/j.gpb.2013.01.003 Text en © 2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Published by Elsevier Ltd and Science Press. All rights reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Review
Chen, Fei
Dong, Mengxing
Ge, Meng
Zhu, Lingxiang
Ren, Lufeng
Liu, Guocheng
Mu, Rong
The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
title The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
title_full The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
title_fullStr The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
title_full_unstemmed The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
title_short The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
title_sort history and advances of reversible terminators used in new generations of sequencing technology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357665/
https://www.ncbi.nlm.nih.gov/pubmed/23414612
http://dx.doi.org/10.1016/j.gpb.2013.01.003
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