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The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology
DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and w...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357665/ https://www.ncbi.nlm.nih.gov/pubmed/23414612 http://dx.doi.org/10.1016/j.gpb.2013.01.003 |
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author | Chen, Fei Dong, Mengxing Ge, Meng Zhu, Lingxiang Ren, Lufeng Liu, Guocheng Mu, Rong |
author_facet | Chen, Fei Dong, Mengxing Ge, Meng Zhu, Lingxiang Ren, Lufeng Liu, Guocheng Mu, Rong |
author_sort | Chen, Fei |
collection | PubMed |
description | DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the “REAP” method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application. |
format | Online Article Text |
id | pubmed-4357665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-43576652015-05-06 The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology Chen, Fei Dong, Mengxing Ge, Meng Zhu, Lingxiang Ren, Lufeng Liu, Guocheng Mu, Rong Genomics Proteomics Bioinformatics Review DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the “REAP” method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application. Elsevier 2013-02 2013-01-23 /pmc/articles/PMC4357665/ /pubmed/23414612 http://dx.doi.org/10.1016/j.gpb.2013.01.003 Text en © 2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Published by Elsevier Ltd and Science Press. All rights reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Review Chen, Fei Dong, Mengxing Ge, Meng Zhu, Lingxiang Ren, Lufeng Liu, Guocheng Mu, Rong The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
title | The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
title_full | The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
title_fullStr | The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
title_full_unstemmed | The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
title_short | The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
title_sort | history and advances of reversible terminators used in new generations of sequencing technology |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357665/ https://www.ncbi.nlm.nih.gov/pubmed/23414612 http://dx.doi.org/10.1016/j.gpb.2013.01.003 |
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