High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity

The chain of events leading from a toxic compound exposure to carcinogenicity is still barely understood. With the emergence of high-throughput sequencing, it is now possible to discover many different biological components simultaneously. Using two different RNA libraries, we sequenced the complete...

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Detalles Bibliográficos
Autores principales: Caiment, Florian, Gaj, Stan, Claessen, Sandra, Kleinjans, Jos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Data Resources and Analyses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357716/
https://www.ncbi.nlm.nih.gov/pubmed/25690898
http://dx.doi.org/10.1093/nar/gkv115
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author Caiment, Florian
Gaj, Stan
Claessen, Sandra
Kleinjans, Jos
author_facet Caiment, Florian
Gaj, Stan
Claessen, Sandra
Kleinjans, Jos
author_sort Caiment, Florian
collection PubMed
description The chain of events leading from a toxic compound exposure to carcinogenicity is still barely understood. With the emergence of high-throughput sequencing, it is now possible to discover many different biological components simultaneously. Using two different RNA libraries, we sequenced the complete transcriptome of human HepG2 liver cells exposed to benzo[a]pyrene, a potent human carcinogen, across six time points. Data were integrated in order to reveal novel complex chemical–gene interactions. Notably, we hypothesized that the inhibition of MGMT, a DNA damage response enzyme, by the over-expressed miR-181a-1_3p induced by BaP, may lead to liver cancer over time.
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spelling pubmed-43577162015-03-20 High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity Caiment, Florian Gaj, Stan Claessen, Sandra Kleinjans, Jos Nucleic Acids Res Data Resources and Analyses The chain of events leading from a toxic compound exposure to carcinogenicity is still barely understood. With the emergence of high-throughput sequencing, it is now possible to discover many different biological components simultaneously. Using two different RNA libraries, we sequenced the complete transcriptome of human HepG2 liver cells exposed to benzo[a]pyrene, a potent human carcinogen, across six time points. Data were integrated in order to reveal novel complex chemical–gene interactions. Notably, we hypothesized that the inhibition of MGMT, a DNA damage response enzyme, by the over-expressed miR-181a-1_3p induced by BaP, may lead to liver cancer over time. Oxford University Press 2015-03-11 2015-02-17 /pmc/articles/PMC4357716/ /pubmed/25690898 http://dx.doi.org/10.1093/nar/gkv115 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Data Resources and Analyses
Caiment, Florian
Gaj, Stan
Claessen, Sandra
Kleinjans, Jos
High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
title High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
title_full High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
title_fullStr High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
title_full_unstemmed High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
title_short High-throughput data integration of RNA–miRNA–circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
title_sort high-throughput data integration of rna–mirna–circrna reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity
topic Data Resources and Analyses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357716/
https://www.ncbi.nlm.nih.gov/pubmed/25690898
http://dx.doi.org/10.1093/nar/gkv115
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