Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells

Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using...

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Detalles Bibliográficos
Autores principales: Wang, Juan, Gu, Qi, Hao, Jie, Bai, Donghui, Liu, Lei, Zhao, Xiaoyang, Liu, Zhonghua, Wang, Liu, Zhou, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357789/
https://www.ncbi.nlm.nih.gov/pubmed/24080286
http://dx.doi.org/10.1016/j.gpb.2013.08.002
Descripción
Sumario:Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.

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