Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells

Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using...

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Detalles Bibliográficos
Autores principales: Wang, Juan, Gu, Qi, Hao, Jie, Bai, Donghui, Liu, Lei, Zhao, Xiaoyang, Liu, Zhonghua, Wang, Liu, Zhou, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357789/
https://www.ncbi.nlm.nih.gov/pubmed/24080286
http://dx.doi.org/10.1016/j.gpb.2013.08.002
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author Wang, Juan
Gu, Qi
Hao, Jie
Bai, Donghui
Liu, Lei
Zhao, Xiaoyang
Liu, Zhonghua
Wang, Liu
Zhou, Qi
author_facet Wang, Juan
Gu, Qi
Hao, Jie
Bai, Donghui
Liu, Lei
Zhao, Xiaoyang
Liu, Zhonghua
Wang, Liu
Zhou, Qi
author_sort Wang, Juan
collection PubMed
description Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.
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spelling pubmed-43577892015-05-06 Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells Wang, Juan Gu, Qi Hao, Jie Bai, Donghui Liu, Lei Zhao, Xiaoyang Liu, Zhonghua Wang, Liu Zhou, Qi Genomics Proteomics Bioinformatics Original Research Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases. Elsevier 2013-10 2013-09-28 /pmc/articles/PMC4357789/ /pubmed/24080286 http://dx.doi.org/10.1016/j.gpb.2013.08.002 Text en © 2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Production and hosting by Elsevier B.V. All rights reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Original Research
Wang, Juan
Gu, Qi
Hao, Jie
Bai, Donghui
Liu, Lei
Zhao, Xiaoyang
Liu, Zhonghua
Wang, Liu
Zhou, Qi
Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
title Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
title_full Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
title_fullStr Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
title_full_unstemmed Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
title_short Generation of Induced Pluripotent Stem Cells with High Efficiency from Human Umbilical Cord Blood Mononuclear Cells
title_sort generation of induced pluripotent stem cells with high efficiency from human umbilical cord blood mononuclear cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357789/
https://www.ncbi.nlm.nih.gov/pubmed/24080286
http://dx.doi.org/10.1016/j.gpb.2013.08.002
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