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Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha
Pores in fungal septa enable cytoplasmic streaming between hyphae and their compartments. Consequently, the mycelium can be considered unicellular. However, we show here that Woronin bodies close ~50% of the three most apical septa of growing hyphae of Aspergillus niger. The incidence of closure of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357997/ https://www.ncbi.nlm.nih.gov/pubmed/25736883 http://dx.doi.org/10.1128/mBio.00111-15 |
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author | Bleichrodt, Robert-Jan Hulsman, Marc Wösten, Han A. B. Reinders, Marcel J. T. |
author_facet | Bleichrodt, Robert-Jan Hulsman, Marc Wösten, Han A. B. Reinders, Marcel J. T. |
author_sort | Bleichrodt, Robert-Jan |
collection | PubMed |
description | Pores in fungal septa enable cytoplasmic streaming between hyphae and their compartments. Consequently, the mycelium can be considered unicellular. However, we show here that Woronin bodies close ~50% of the three most apical septa of growing hyphae of Aspergillus niger. The incidence of closure of the 9th and 10th septa was even ≥94%. Intercompartmental streaming of photoactivatable green fluorescent protein (PA-GFP) was not observed when the septa were closed, but open septa acted as a barrier, reducing the mobility rate of PA-GFP ~500 times. This mobility rate decreased with increasing septal age and under stress conditions, likely reflecting a regulatory mechanism affecting septal pore diameter. Modeling revealed that such regulation offers effective control of compound concentration between compartments. Modeling also showed that the incidence of septal closure in A. niger had an even stronger impact on cytoplasmic continuity. Cytoplasm of hyphal compartments was shown not to be in physical contact when separated by more than 4 septa. Together, data show that apical compartments of growing hyphae behave unicellularly, while older compartments have a multicellular organization. |
format | Online Article Text |
id | pubmed-4357997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-43579972015-03-17 Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha Bleichrodt, Robert-Jan Hulsman, Marc Wösten, Han A. B. Reinders, Marcel J. T. mBio Research Article Pores in fungal septa enable cytoplasmic streaming between hyphae and their compartments. Consequently, the mycelium can be considered unicellular. However, we show here that Woronin bodies close ~50% of the three most apical septa of growing hyphae of Aspergillus niger. The incidence of closure of the 9th and 10th septa was even ≥94%. Intercompartmental streaming of photoactivatable green fluorescent protein (PA-GFP) was not observed when the septa were closed, but open septa acted as a barrier, reducing the mobility rate of PA-GFP ~500 times. This mobility rate decreased with increasing septal age and under stress conditions, likely reflecting a regulatory mechanism affecting septal pore diameter. Modeling revealed that such regulation offers effective control of compound concentration between compartments. Modeling also showed that the incidence of septal closure in A. niger had an even stronger impact on cytoplasmic continuity. Cytoplasm of hyphal compartments was shown not to be in physical contact when separated by more than 4 septa. Together, data show that apical compartments of growing hyphae behave unicellularly, while older compartments have a multicellular organization. American Society of Microbiology 2015-03-03 /pmc/articles/PMC4357997/ /pubmed/25736883 http://dx.doi.org/10.1128/mBio.00111-15 Text en Copyright © 2015 Bleichrodt et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bleichrodt, Robert-Jan Hulsman, Marc Wösten, Han A. B. Reinders, Marcel J. T. Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha |
title | Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha |
title_full | Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha |
title_fullStr | Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha |
title_full_unstemmed | Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha |
title_short | Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha |
title_sort | switching from a unicellular to multicellular organization in an aspergillus niger hypha |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357997/ https://www.ncbi.nlm.nih.gov/pubmed/25736883 http://dx.doi.org/10.1128/mBio.00111-15 |
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