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Biosynthesis of soluble carotenoid holoproteins in Escherichia coli
Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358027/ https://www.ncbi.nlm.nih.gov/pubmed/25765842 http://dx.doi.org/10.1038/srep09085 |
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author | de Carbon, Céline Bourcier Thurotte, Adrien Wilson, Adjélé Perreau, François Kirilovsky, Diana |
author_facet | de Carbon, Céline Bourcier Thurotte, Adrien Wilson, Adjélé Perreau, François Kirilovsky, Diana |
author_sort | de Carbon, Céline Bourcier |
collection | PubMed |
description | Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in E.coli. The six different genes involved in the synthesis of holo-OCP were introduced into E. coli using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the ocp gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. This approach for rapid heterologous synthesis of large quantities of carotenoproteins is a fundamental advance in the production of antioxidants of great interest to the pharmaceutical and cosmetic industries. |
format | Online Article Text |
id | pubmed-4358027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43580272015-03-17 Biosynthesis of soluble carotenoid holoproteins in Escherichia coli de Carbon, Céline Bourcier Thurotte, Adrien Wilson, Adjélé Perreau, François Kirilovsky, Diana Sci Rep Article Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in E.coli. The six different genes involved in the synthesis of holo-OCP were introduced into E. coli using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the ocp gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. This approach for rapid heterologous synthesis of large quantities of carotenoproteins is a fundamental advance in the production of antioxidants of great interest to the pharmaceutical and cosmetic industries. Nature Publishing Group 2015-03-13 /pmc/articles/PMC4358027/ /pubmed/25765842 http://dx.doi.org/10.1038/srep09085 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article de Carbon, Céline Bourcier Thurotte, Adrien Wilson, Adjélé Perreau, François Kirilovsky, Diana Biosynthesis of soluble carotenoid holoproteins in Escherichia coli |
title | Biosynthesis of soluble carotenoid holoproteins in Escherichia coli |
title_full | Biosynthesis of soluble carotenoid holoproteins in Escherichia coli |
title_fullStr | Biosynthesis of soluble carotenoid holoproteins in Escherichia coli |
title_full_unstemmed | Biosynthesis of soluble carotenoid holoproteins in Escherichia coli |
title_short | Biosynthesis of soluble carotenoid holoproteins in Escherichia coli |
title_sort | biosynthesis of soluble carotenoid holoproteins in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358027/ https://www.ncbi.nlm.nih.gov/pubmed/25765842 http://dx.doi.org/10.1038/srep09085 |
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