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The effects of kisspeptin-10 on migration and proliferation of endothelial cell
BACKGROUND: Migration, expansion and survival of endothelial cells that are an important cellular component of blood vessels plays an important role in the induction of tumor growth. Kisspeptins (kp), peptides that bind to coupled-G protein receptor (GPR54), inhibit each step of metastatic cascade i...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358036/ https://www.ncbi.nlm.nih.gov/pubmed/25789267 http://dx.doi.org/10.4103/2277-9175.151250 |
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author | Golzar, Fatemeh Javanmard, Shaghayegh Haghjooy |
author_facet | Golzar, Fatemeh Javanmard, Shaghayegh Haghjooy |
author_sort | Golzar, Fatemeh |
collection | PubMed |
description | BACKGROUND: Migration, expansion and survival of endothelial cells that are an important cellular component of blood vessels plays an important role in the induction of tumor growth. Kisspeptins (kp), peptides that bind to coupled-G protein receptor (GPR54), inhibit each step of metastatic cascade include invasion, migration and homing, angiogenesis, survival and proliferation. In this study we investigated effects of kisspeptin-10, the most potent member of kisspeptin family, on Migration and proliferation of endothelial cells that are necessary for angiogenesis and tumor metastasis. MATERIALS AND METHODS: We compared migration of Human Umbilical Vein Endothelial Cells (HUVECs) were treated with 10-100 or 500 nM kp-10 for 24 hours and no treated cells using an in vitro trans membrane migration assay and HUVEC proliferation of treated endothelial cells with 10-100 or 500 nM kp-10 for 48 hours and no treated cells was measured by MTT Cell Proliferation Assay Kit. Analysis of data was performed using the Kruskal-Wallis test followed by the Mann-Whitney test. RESULTS: Migration and proliferation of endothelial cells were increased at lower concentration of kp-10 specially at 100 nM while higher concentration reduced both migration and proliferation. CONCLUSION: Our data showed that different concentrations of kp-10 have distinct effects on migration and proliferation of endothelial cells. |
format | Online Article Text |
id | pubmed-4358036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43580362015-03-18 The effects of kisspeptin-10 on migration and proliferation of endothelial cell Golzar, Fatemeh Javanmard, Shaghayegh Haghjooy Adv Biomed Res Original Article BACKGROUND: Migration, expansion and survival of endothelial cells that are an important cellular component of blood vessels plays an important role in the induction of tumor growth. Kisspeptins (kp), peptides that bind to coupled-G protein receptor (GPR54), inhibit each step of metastatic cascade include invasion, migration and homing, angiogenesis, survival and proliferation. In this study we investigated effects of kisspeptin-10, the most potent member of kisspeptin family, on Migration and proliferation of endothelial cells that are necessary for angiogenesis and tumor metastasis. MATERIALS AND METHODS: We compared migration of Human Umbilical Vein Endothelial Cells (HUVECs) were treated with 10-100 or 500 nM kp-10 for 24 hours and no treated cells using an in vitro trans membrane migration assay and HUVEC proliferation of treated endothelial cells with 10-100 or 500 nM kp-10 for 48 hours and no treated cells was measured by MTT Cell Proliferation Assay Kit. Analysis of data was performed using the Kruskal-Wallis test followed by the Mann-Whitney test. RESULTS: Migration and proliferation of endothelial cells were increased at lower concentration of kp-10 specially at 100 nM while higher concentration reduced both migration and proliferation. CONCLUSION: Our data showed that different concentrations of kp-10 have distinct effects on migration and proliferation of endothelial cells. Medknow Publications & Media Pvt Ltd 2015-02-11 /pmc/articles/PMC4358036/ /pubmed/25789267 http://dx.doi.org/10.4103/2277-9175.151250 Text en Copyright: © 2015 Golzar F. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Original Article Golzar, Fatemeh Javanmard, Shaghayegh Haghjooy The effects of kisspeptin-10 on migration and proliferation of endothelial cell |
title | The effects of kisspeptin-10 on migration and proliferation of endothelial cell |
title_full | The effects of kisspeptin-10 on migration and proliferation of endothelial cell |
title_fullStr | The effects of kisspeptin-10 on migration and proliferation of endothelial cell |
title_full_unstemmed | The effects of kisspeptin-10 on migration and proliferation of endothelial cell |
title_short | The effects of kisspeptin-10 on migration and proliferation of endothelial cell |
title_sort | effects of kisspeptin-10 on migration and proliferation of endothelial cell |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358036/ https://www.ncbi.nlm.nih.gov/pubmed/25789267 http://dx.doi.org/10.4103/2277-9175.151250 |
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