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Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strai...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358045/ https://www.ncbi.nlm.nih.gov/pubmed/25765923 http://dx.doi.org/10.1038/srep09086 |
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author | Cabañes, F. Javier Sanseverino, Walter Castellá, Gemma Bragulat, M. Rosa Cigliano, Riccardo Aiese Sánchez, Armand |
author_facet | Cabañes, F. Javier Sanseverino, Walter Castellá, Gemma Bragulat, M. Rosa Cigliano, Riccardo Aiese Sánchez, Armand |
author_sort | Cabañes, F. Javier |
collection | PubMed |
description | In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis. |
format | Online Article Text |
id | pubmed-4358045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43580452015-03-17 Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius Cabañes, F. Javier Sanseverino, Walter Castellá, Gemma Bragulat, M. Rosa Cigliano, Riccardo Aiese Sánchez, Armand Sci Rep Article In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis. Nature Publishing Group 2015-03-13 /pmc/articles/PMC4358045/ /pubmed/25765923 http://dx.doi.org/10.1038/srep09086 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Cabañes, F. Javier Sanseverino, Walter Castellá, Gemma Bragulat, M. Rosa Cigliano, Riccardo Aiese Sánchez, Armand Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius |
title | Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius |
title_full | Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius |
title_fullStr | Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius |
title_full_unstemmed | Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius |
title_short | Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius |
title_sort | rapid genome resequencing of an atoxigenic strain of aspergillus carbonarius |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358045/ https://www.ncbi.nlm.nih.gov/pubmed/25765923 http://dx.doi.org/10.1038/srep09086 |
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