Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides

Development of angiotensin I-converting enzyme (ACE, EC 3.4.15.1) inhibitory peptides from food protein is under extensive research as alternative for the prevention of hypertension. However, it is difficult to identify peptides released from food sources. To accelerate the progress of peptide ident...

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Detalles Bibliográficos
Autores principales: Zhang, Ting, Nie, Shaoping, Liu, Boqun, Yu, Yiding, Zhang, Yan, Liu, Jingbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358945/
https://www.ncbi.nlm.nih.gov/pubmed/25768442
http://dx.doi.org/10.1371/journal.pone.0119598
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author Zhang, Ting
Nie, Shaoping
Liu, Boqun
Yu, Yiding
Zhang, Yan
Liu, Jingbo
author_facet Zhang, Ting
Nie, Shaoping
Liu, Boqun
Yu, Yiding
Zhang, Yan
Liu, Jingbo
author_sort Zhang, Ting
collection PubMed
description Development of angiotensin I-converting enzyme (ACE, EC 3.4.15.1) inhibitory peptides from food protein is under extensive research as alternative for the prevention of hypertension. However, it is difficult to identify peptides released from food sources. To accelerate the progress of peptide identification, a three layer back propagation neural network model was established to predict the ACE-inhibitory activity of pentapeptides derived from bovine hemoglobin by simulated enzyme digestion. The pentapeptide WTQRF has the best predicted value with experimental IC(50) 23.93 μM. The potential molecular mechanism of the WTQRF / ACE interaction was investigated by flexible docking.
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spelling pubmed-43589452015-03-23 Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides Zhang, Ting Nie, Shaoping Liu, Boqun Yu, Yiding Zhang, Yan Liu, Jingbo PLoS One Research Article Development of angiotensin I-converting enzyme (ACE, EC 3.4.15.1) inhibitory peptides from food protein is under extensive research as alternative for the prevention of hypertension. However, it is difficult to identify peptides released from food sources. To accelerate the progress of peptide identification, a three layer back propagation neural network model was established to predict the ACE-inhibitory activity of pentapeptides derived from bovine hemoglobin by simulated enzyme digestion. The pentapeptide WTQRF has the best predicted value with experimental IC(50) 23.93 μM. The potential molecular mechanism of the WTQRF / ACE interaction was investigated by flexible docking. Public Library of Science 2015-03-13 /pmc/articles/PMC4358945/ /pubmed/25768442 http://dx.doi.org/10.1371/journal.pone.0119598 Text en © 2015 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Ting
Nie, Shaoping
Liu, Boqun
Yu, Yiding
Zhang, Yan
Liu, Jingbo
Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides
title Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides
title_full Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides
title_fullStr Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides
title_full_unstemmed Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides
title_short Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides
title_sort activity prediction and molecular mechanism of bovine blood derived angiotensin i-converting enzyme inhibitory peptides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358945/
https://www.ncbi.nlm.nih.gov/pubmed/25768442
http://dx.doi.org/10.1371/journal.pone.0119598
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