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Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation
The long-term potentiation (LTP) of spinal C-fiber-evoked field potentials is considered as a fundamental mechanism of central sensitization in the spinal cord. Accumulating evidence has showed the contribution of spinal microglia to spinal LTP and pathological pain. As a key signaling of neurons-mi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358970/ https://www.ncbi.nlm.nih.gov/pubmed/25768734 http://dx.doi.org/10.1371/journal.pone.0118842 |
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author | Bian, Chao Zhao, Zhi-Qi Zhang, Yu-Qiu Lü, Ning |
author_facet | Bian, Chao Zhao, Zhi-Qi Zhang, Yu-Qiu Lü, Ning |
author_sort | Bian, Chao |
collection | PubMed |
description | The long-term potentiation (LTP) of spinal C-fiber-evoked field potentials is considered as a fundamental mechanism of central sensitization in the spinal cord. Accumulating evidence has showed the contribution of spinal microglia to spinal LTP and pathological pain. As a key signaling of neurons-microglia interactions, the involvement of CX3CL1/CX3CR1 signaling in pathological pain has also been investigated extensively. The present study examined whether CX3CL1/CX3CR1 signaling plays a role in spinal LTP. The results showed that 10-trains tetanic stimulation (100 Hz, 2s) of the sciatic nerve (TSS) produced a significant LTP of C-fiber-evoked field potentials lasting for over 3 h in the rat spinal dorsal horn. Blockade of CX3CL1/CX3CR1 signaling with an anti-CX3CR1 neutralizing antibody (CX3CR1 AB) markedly suppressed TSS-induced LTP. Exogenous CX3CL1 significantly potentiated 3-trains TSS-induced LTP in rats. Consistently, spinal LTP of C-fiber-evoked field potentials was also induced by TSS (100 Hz, 1s, 4 trains) in all C57BL/6 wild type (WT) mice. However, in CX3CR1(-/-) mice, TSS failed to induce LTP and behavioral hypersensitivity, confirming an essential role of CX3CR1 in spinal LTP induction. Furthermore, blockade of IL-18 or IL-23, the potential downstream factors of CX3CL1/CX3CR1 signaling, with IL-18 BP or anti-IL-23 neutralizing antibody (IL-23 AB), obviously suppressed spinal LTP in rats. These results suggest that CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn. |
format | Online Article Text |
id | pubmed-4358970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43589702015-03-23 Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation Bian, Chao Zhao, Zhi-Qi Zhang, Yu-Qiu Lü, Ning PLoS One Research Article The long-term potentiation (LTP) of spinal C-fiber-evoked field potentials is considered as a fundamental mechanism of central sensitization in the spinal cord. Accumulating evidence has showed the contribution of spinal microglia to spinal LTP and pathological pain. As a key signaling of neurons-microglia interactions, the involvement of CX3CL1/CX3CR1 signaling in pathological pain has also been investigated extensively. The present study examined whether CX3CL1/CX3CR1 signaling plays a role in spinal LTP. The results showed that 10-trains tetanic stimulation (100 Hz, 2s) of the sciatic nerve (TSS) produced a significant LTP of C-fiber-evoked field potentials lasting for over 3 h in the rat spinal dorsal horn. Blockade of CX3CL1/CX3CR1 signaling with an anti-CX3CR1 neutralizing antibody (CX3CR1 AB) markedly suppressed TSS-induced LTP. Exogenous CX3CL1 significantly potentiated 3-trains TSS-induced LTP in rats. Consistently, spinal LTP of C-fiber-evoked field potentials was also induced by TSS (100 Hz, 1s, 4 trains) in all C57BL/6 wild type (WT) mice. However, in CX3CR1(-/-) mice, TSS failed to induce LTP and behavioral hypersensitivity, confirming an essential role of CX3CR1 in spinal LTP induction. Furthermore, blockade of IL-18 or IL-23, the potential downstream factors of CX3CL1/CX3CR1 signaling, with IL-18 BP or anti-IL-23 neutralizing antibody (IL-23 AB), obviously suppressed spinal LTP in rats. These results suggest that CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn. Public Library of Science 2015-03-13 /pmc/articles/PMC4358970/ /pubmed/25768734 http://dx.doi.org/10.1371/journal.pone.0118842 Text en © 2015 Bian et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bian, Chao Zhao, Zhi-Qi Zhang, Yu-Qiu Lü, Ning Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation |
title | Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation |
title_full | Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation |
title_fullStr | Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation |
title_full_unstemmed | Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation |
title_short | Involvement of CX3CL1/CX3CR1 Signaling in Spinal Long Term Potentiation |
title_sort | involvement of cx3cl1/cx3cr1 signaling in spinal long term potentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358970/ https://www.ncbi.nlm.nih.gov/pubmed/25768734 http://dx.doi.org/10.1371/journal.pone.0118842 |
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