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Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome

The genome assembly of southern house mosquito Cx. quinquefasciatus is represented by a high number of supercontigs with no order or orientation on the chromosomes. Although cytogenetic maps for the polytene chromosomes of this mosquito have been developed, their utilization for the genome mapping r...

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Autores principales: Naumenko, Anastasia N., Timoshevskiy, Vladimir A., Kinney, Nicholas A., Kokhanenko, Alina A., deBruyn, Becky S., Lovin, Diane D., Stegniy, Vladimir N., Severson, David W., Sharakhov, Igor V., Sharakhova, Maria V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358980/
https://www.ncbi.nlm.nih.gov/pubmed/25768920
http://dx.doi.org/10.1371/journal.pone.0115737
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author Naumenko, Anastasia N.
Timoshevskiy, Vladimir A.
Kinney, Nicholas A.
Kokhanenko, Alina A.
deBruyn, Becky S.
Lovin, Diane D.
Stegniy, Vladimir N.
Severson, David W.
Sharakhov, Igor V.
Sharakhova, Maria V.
author_facet Naumenko, Anastasia N.
Timoshevskiy, Vladimir A.
Kinney, Nicholas A.
Kokhanenko, Alina A.
deBruyn, Becky S.
Lovin, Diane D.
Stegniy, Vladimir N.
Severson, David W.
Sharakhov, Igor V.
Sharakhova, Maria V.
author_sort Naumenko, Anastasia N.
collection PubMed
description The genome assembly of southern house mosquito Cx. quinquefasciatus is represented by a high number of supercontigs with no order or orientation on the chromosomes. Although cytogenetic maps for the polytene chromosomes of this mosquito have been developed, their utilization for the genome mapping remains difficult because of the low number of high-quality spreads in chromosome preparations. Therefore, a simple and robust mitotic-chromosome-based approach for the genome mapping of Cx. quinquefasciatus still needs to be developed. In this study, we performed physical mapping of 37 genomic supercontigs using fluorescent in situ hybridization on mitotic chromosomes from imaginal discs of 4th instar larvae. The genetic linkage map nomenclature was adopted for the chromosome numbering based on the direct positioning of 58 markers that were previously genetically mapped. The smallest, largest, and intermediate chromosomes were numbered as 1, 2, and 3, respectively. For idiogram development, we analyzed and described in detail the morphology and proportions of the mitotic chromosomes. Chromosomes were subdivided into 19 divisions and 72 bands of four different intensities. These idiograms were used for mapping the genomic supercontigs/genetic markers. We also determined the presence of length polymorphism in the q arm of sex-determining chromosome 1 in Cx. quinquefasciatus related to the size of ribosomal locus. Our physical mapping and previous genetic linkage mapping resulted in the chromosomal assignment of 13% of the total genome assembly to the chromosome bands. We provided the first detailed description, nomenclature, and idiograms for the mitotic chromosomes of Cx. quinquefasciatus. Further application of the approach developed in this study will help to improve the quality of the southern house mosquito genome.
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spelling pubmed-43589802015-03-23 Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome Naumenko, Anastasia N. Timoshevskiy, Vladimir A. Kinney, Nicholas A. Kokhanenko, Alina A. deBruyn, Becky S. Lovin, Diane D. Stegniy, Vladimir N. Severson, David W. Sharakhov, Igor V. Sharakhova, Maria V. PLoS One Research Article The genome assembly of southern house mosquito Cx. quinquefasciatus is represented by a high number of supercontigs with no order or orientation on the chromosomes. Although cytogenetic maps for the polytene chromosomes of this mosquito have been developed, their utilization for the genome mapping remains difficult because of the low number of high-quality spreads in chromosome preparations. Therefore, a simple and robust mitotic-chromosome-based approach for the genome mapping of Cx. quinquefasciatus still needs to be developed. In this study, we performed physical mapping of 37 genomic supercontigs using fluorescent in situ hybridization on mitotic chromosomes from imaginal discs of 4th instar larvae. The genetic linkage map nomenclature was adopted for the chromosome numbering based on the direct positioning of 58 markers that were previously genetically mapped. The smallest, largest, and intermediate chromosomes were numbered as 1, 2, and 3, respectively. For idiogram development, we analyzed and described in detail the morphology and proportions of the mitotic chromosomes. Chromosomes were subdivided into 19 divisions and 72 bands of four different intensities. These idiograms were used for mapping the genomic supercontigs/genetic markers. We also determined the presence of length polymorphism in the q arm of sex-determining chromosome 1 in Cx. quinquefasciatus related to the size of ribosomal locus. Our physical mapping and previous genetic linkage mapping resulted in the chromosomal assignment of 13% of the total genome assembly to the chromosome bands. We provided the first detailed description, nomenclature, and idiograms for the mitotic chromosomes of Cx. quinquefasciatus. Further application of the approach developed in this study will help to improve the quality of the southern house mosquito genome. Public Library of Science 2015-03-13 /pmc/articles/PMC4358980/ /pubmed/25768920 http://dx.doi.org/10.1371/journal.pone.0115737 Text en © 2015 Naumenko et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Naumenko, Anastasia N.
Timoshevskiy, Vladimir A.
Kinney, Nicholas A.
Kokhanenko, Alina A.
deBruyn, Becky S.
Lovin, Diane D.
Stegniy, Vladimir N.
Severson, David W.
Sharakhov, Igor V.
Sharakhova, Maria V.
Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome
title Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome
title_full Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome
title_fullStr Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome
title_full_unstemmed Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome
title_short Mitotic-Chromosome-Based Physical Mapping of the Culex quinquefasciatus Genome
title_sort mitotic-chromosome-based physical mapping of the culex quinquefasciatus genome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358980/
https://www.ncbi.nlm.nih.gov/pubmed/25768920
http://dx.doi.org/10.1371/journal.pone.0115737
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