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Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates spacer acquisition in vivo, but the molecular mechanism of this process is unknown. Here we show that the purified Cas1–Cas2 comp...

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Detalles Bibliográficos
Autores principales:	Nuñez, James K., Lee, Amy S.Y., Engelman, Alan, Doudna, Jennifer A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359072/ https://www.ncbi.nlm.nih.gov/pubmed/25707795 http://dx.doi.org/10.1038/nature14237

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