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Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions

In mosquitoes, the maternally inherited bacterial Wolbachia induce a form of embryonic lethality called cytoplasmic incompatibility (CI). This property can be used to reduce the density of mosquito field populations through inundative releases of incompatible males in order to sterilize females (Inc...

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Autores principales: Atyame, Célestine M., Cattel, Julien, Lebon, Cyrille, Flores, Olivier, Dehecq, Jean-Sébastien, Weill, Mylène, Gouagna, Louis Clément, Tortosa, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359102/
https://www.ncbi.nlm.nih.gov/pubmed/25768841
http://dx.doi.org/10.1371/journal.pone.0119288
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author Atyame, Célestine M.
Cattel, Julien
Lebon, Cyrille
Flores, Olivier
Dehecq, Jean-Sébastien
Weill, Mylène
Gouagna, Louis Clément
Tortosa, Pablo
author_facet Atyame, Célestine M.
Cattel, Julien
Lebon, Cyrille
Flores, Olivier
Dehecq, Jean-Sébastien
Weill, Mylène
Gouagna, Louis Clément
Tortosa, Pablo
author_sort Atyame, Célestine M.
collection PubMed
description In mosquitoes, the maternally inherited bacterial Wolbachia induce a form of embryonic lethality called cytoplasmic incompatibility (CI). This property can be used to reduce the density of mosquito field populations through inundative releases of incompatible males in order to sterilize females (Incompatible Insect Technique, or IIT, strategy). We have previously constructed the LR[wPip(Is)] line representing a good candidate for controlling field populations of the Culex quinquefasciatus mosquito in the islands of the south-western Indian Ocean. The main purpose of the present study was to fill the gap between laboratory experiments and field implementation, i.e. assessing mating competitiveness of these incompatible males under semi-field conditions. In a first set of experiments, we analyzed crossing relationships between LR[wPip(Is)] males and La Réunion field females collected as larvae in 19 distinct localities throughout the island. This investigation revealed total embryonic mortality, confirming the strong sterilizing capacity of LR[wPip(Is)] males. Subsequently, mating competitiveness of LR[wPip(Is)] males was assessed under semi-field conditions in the presence of field males and females from La Réunion. Confrontations were carried out in April and December using different ratios of LR[wPip(Is)] to field males. The results indicated that the LR[wPip(Is)] males successfully compete with field males in mating with field females, displaying even higher competitiveness than field males in April. Our results support the implementation of small-scale field tests in order to assess the feasibility of IIT against Cx. quinquefasciatus in the islands of southwestern Indian Ocean where this mosquito species is a proven competent vector for human pathogens.
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spelling pubmed-43591022015-03-23 Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions Atyame, Célestine M. Cattel, Julien Lebon, Cyrille Flores, Olivier Dehecq, Jean-Sébastien Weill, Mylène Gouagna, Louis Clément Tortosa, Pablo PLoS One Research Article In mosquitoes, the maternally inherited bacterial Wolbachia induce a form of embryonic lethality called cytoplasmic incompatibility (CI). This property can be used to reduce the density of mosquito field populations through inundative releases of incompatible males in order to sterilize females (Incompatible Insect Technique, or IIT, strategy). We have previously constructed the LR[wPip(Is)] line representing a good candidate for controlling field populations of the Culex quinquefasciatus mosquito in the islands of the south-western Indian Ocean. The main purpose of the present study was to fill the gap between laboratory experiments and field implementation, i.e. assessing mating competitiveness of these incompatible males under semi-field conditions. In a first set of experiments, we analyzed crossing relationships between LR[wPip(Is)] males and La Réunion field females collected as larvae in 19 distinct localities throughout the island. This investigation revealed total embryonic mortality, confirming the strong sterilizing capacity of LR[wPip(Is)] males. Subsequently, mating competitiveness of LR[wPip(Is)] males was assessed under semi-field conditions in the presence of field males and females from La Réunion. Confrontations were carried out in April and December using different ratios of LR[wPip(Is)] to field males. The results indicated that the LR[wPip(Is)] males successfully compete with field males in mating with field females, displaying even higher competitiveness than field males in April. Our results support the implementation of small-scale field tests in order to assess the feasibility of IIT against Cx. quinquefasciatus in the islands of southwestern Indian Ocean where this mosquito species is a proven competent vector for human pathogens. Public Library of Science 2015-03-13 /pmc/articles/PMC4359102/ /pubmed/25768841 http://dx.doi.org/10.1371/journal.pone.0119288 Text en © 2015 Atyame et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Atyame, Célestine M.
Cattel, Julien
Lebon, Cyrille
Flores, Olivier
Dehecq, Jean-Sébastien
Weill, Mylène
Gouagna, Louis Clément
Tortosa, Pablo
Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions
title Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions
title_full Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions
title_fullStr Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions
title_full_unstemmed Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions
title_short Wolbachia-Based Population Control Strategy Targeting Culex quinquefasciatus Mosquitoes Proves Efficient under Semi-Field Conditions
title_sort wolbachia-based population control strategy targeting culex quinquefasciatus mosquitoes proves efficient under semi-field conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359102/
https://www.ncbi.nlm.nih.gov/pubmed/25768841
http://dx.doi.org/10.1371/journal.pone.0119288
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