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IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli
Systemic infections by avian pathogenic Escherichia coli (APEC) are economically devastating to poultry industries worldwide. IbeR, located on genomic island GimA, was shown to serve as an RpoS-like regulator in rpoS gene mutation neonatal meningitis E. coli (NMEC) RS218. However, the role of IbeR i...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359115/ https://www.ncbi.nlm.nih.gov/pubmed/25768126 http://dx.doi.org/10.1371/journal.pone.0119698 |
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author | Wang, Shaohui Bao, Yinli Meng, Qingmei Xia, Yongjie Zhao, Yichao Wang, Yang Tang, Fang ZhuGe, Xiangkai Yu, Shengqing Han, Xiangan Dai, Jianjun Lu, Chengping |
author_facet | Wang, Shaohui Bao, Yinli Meng, Qingmei Xia, Yongjie Zhao, Yichao Wang, Yang Tang, Fang ZhuGe, Xiangkai Yu, Shengqing Han, Xiangan Dai, Jianjun Lu, Chengping |
author_sort | Wang, Shaohui |
collection | PubMed |
description | Systemic infections by avian pathogenic Escherichia coli (APEC) are economically devastating to poultry industries worldwide. IbeR, located on genomic island GimA, was shown to serve as an RpoS-like regulator in rpoS gene mutation neonatal meningitis E. coli (NMEC) RS218. However, the role of IbeR in pathogenicity of APEC carrying active RpoS has not yet been investigated. We showed that the APEC IbeR could elicit antibodies in infected ducks, suggesting that IbeR might be involved in APEC pathogenicity. To investigate the function of IbeR in APEC pathogenesis, mutant and complementation strains were constructed and characterized. Inactivation of ibeR led to attenuated virulence and reduced invasion capacity towards DF-1 cells, brains and cerebrospinal fluid (CSF) in vitro and in vivo. Bactericidal assays demonstrated that the mutant strain had impaired resistance to environmental stress and specific pathogen-free (SPF) chicken serum. These virulence-related phenotypes were restored by genetic complementation. Quantitative real-time reverse transcription PCR revealed that IbeR controlled expression of stress-resistance genes and virulence genes, which might led to the associated virulence phenotype. |
format | Online Article Text |
id | pubmed-4359115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43591152015-03-23 IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli Wang, Shaohui Bao, Yinli Meng, Qingmei Xia, Yongjie Zhao, Yichao Wang, Yang Tang, Fang ZhuGe, Xiangkai Yu, Shengqing Han, Xiangan Dai, Jianjun Lu, Chengping PLoS One Research Article Systemic infections by avian pathogenic Escherichia coli (APEC) are economically devastating to poultry industries worldwide. IbeR, located on genomic island GimA, was shown to serve as an RpoS-like regulator in rpoS gene mutation neonatal meningitis E. coli (NMEC) RS218. However, the role of IbeR in pathogenicity of APEC carrying active RpoS has not yet been investigated. We showed that the APEC IbeR could elicit antibodies in infected ducks, suggesting that IbeR might be involved in APEC pathogenicity. To investigate the function of IbeR in APEC pathogenesis, mutant and complementation strains were constructed and characterized. Inactivation of ibeR led to attenuated virulence and reduced invasion capacity towards DF-1 cells, brains and cerebrospinal fluid (CSF) in vitro and in vivo. Bactericidal assays demonstrated that the mutant strain had impaired resistance to environmental stress and specific pathogen-free (SPF) chicken serum. These virulence-related phenotypes were restored by genetic complementation. Quantitative real-time reverse transcription PCR revealed that IbeR controlled expression of stress-resistance genes and virulence genes, which might led to the associated virulence phenotype. Public Library of Science 2015-03-13 /pmc/articles/PMC4359115/ /pubmed/25768126 http://dx.doi.org/10.1371/journal.pone.0119698 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Shaohui Bao, Yinli Meng, Qingmei Xia, Yongjie Zhao, Yichao Wang, Yang Tang, Fang ZhuGe, Xiangkai Yu, Shengqing Han, Xiangan Dai, Jianjun Lu, Chengping IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli |
title | IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli
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title_full | IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli
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title_fullStr | IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli
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title_full_unstemmed | IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli
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title_short | IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli
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title_sort | iber facilitates stress-resistance, invasion and pathogenicity of avian pathogenic escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359115/ https://www.ncbi.nlm.nih.gov/pubmed/25768126 http://dx.doi.org/10.1371/journal.pone.0119698 |
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