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A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing

C-to-U editing of transcripts in plant organelles is carried out by small (<400 kD) protein complexes called editosomes. Recognition of the proper C target for editing is mediated by pentatricopeptide repeat (PPR) containing proteins that recognize cis-elements. Members of two additional gene fam...

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Autores principales: Sun, Tao, Shi, Xiaowen, Friso, Giulia, Van Wijk, Klaas, Bentolila, Stephane, Hanson, Maureen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359148/
https://www.ncbi.nlm.nih.gov/pubmed/25768119
http://dx.doi.org/10.1371/journal.pgen.1005028
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author Sun, Tao
Shi, Xiaowen
Friso, Giulia
Van Wijk, Klaas
Bentolila, Stephane
Hanson, Maureen R.
author_facet Sun, Tao
Shi, Xiaowen
Friso, Giulia
Van Wijk, Klaas
Bentolila, Stephane
Hanson, Maureen R.
author_sort Sun, Tao
collection PubMed
description C-to-U editing of transcripts in plant organelles is carried out by small (<400 kD) protein complexes called editosomes. Recognition of the proper C target for editing is mediated by pentatricopeptide repeat (PPR) containing proteins that recognize cis-elements. Members of two additional gene families, the RIP/MORF and ORRM families, have each been found to be required for editing of particular sets of Cs in mitochondria and/or chloroplasts. By co-immunoprecipitation of the chloroplast editing factor ORRM1, followed by mass spectrometry, we have now identified a member of the RanBP2 type zinc fingers (pFAM00641) protein family that is required for editing of 14 sites in chloroplasts and affects editing efficiency of another 16 chloroplast C targets. In yeast two-hybrid assays, OZ1 (Organelle Zinc finger 1) interacts with PPR site recognition factors whose cognate sites are affected when OZ1 is mutated. No interaction of OZ1 with the chloroplast editing factors RIP2 and RIP9 was detected; however, OZ1 interacts with ORRM1, which binds to RIP proteins, allowing us to build a model for the chloroplast RNA editosome. The RNA editosomes that act upon most chloroplast C targets are likely to contain a PPR protein recognition factor, either RIP2 or RIP9, ORRM1, and OZ1. The organelle zinc finger editing factor family (OZ) contains 4 members in Arabidopsis, three that are predicted to be targeted to chloroplasts and one to mitochondria. With the identification of OZ1, there are now 4 nuclear-encoded protein families known to be essential for plant organelle RNA editing.
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spelling pubmed-43591482015-03-23 A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing Sun, Tao Shi, Xiaowen Friso, Giulia Van Wijk, Klaas Bentolila, Stephane Hanson, Maureen R. PLoS Genet Research Article C-to-U editing of transcripts in plant organelles is carried out by small (<400 kD) protein complexes called editosomes. Recognition of the proper C target for editing is mediated by pentatricopeptide repeat (PPR) containing proteins that recognize cis-elements. Members of two additional gene families, the RIP/MORF and ORRM families, have each been found to be required for editing of particular sets of Cs in mitochondria and/or chloroplasts. By co-immunoprecipitation of the chloroplast editing factor ORRM1, followed by mass spectrometry, we have now identified a member of the RanBP2 type zinc fingers (pFAM00641) protein family that is required for editing of 14 sites in chloroplasts and affects editing efficiency of another 16 chloroplast C targets. In yeast two-hybrid assays, OZ1 (Organelle Zinc finger 1) interacts with PPR site recognition factors whose cognate sites are affected when OZ1 is mutated. No interaction of OZ1 with the chloroplast editing factors RIP2 and RIP9 was detected; however, OZ1 interacts with ORRM1, which binds to RIP proteins, allowing us to build a model for the chloroplast RNA editosome. The RNA editosomes that act upon most chloroplast C targets are likely to contain a PPR protein recognition factor, either RIP2 or RIP9, ORRM1, and OZ1. The organelle zinc finger editing factor family (OZ) contains 4 members in Arabidopsis, three that are predicted to be targeted to chloroplasts and one to mitochondria. With the identification of OZ1, there are now 4 nuclear-encoded protein families known to be essential for plant organelle RNA editing. Public Library of Science 2015-03-13 /pmc/articles/PMC4359148/ /pubmed/25768119 http://dx.doi.org/10.1371/journal.pgen.1005028 Text en © 2015 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sun, Tao
Shi, Xiaowen
Friso, Giulia
Van Wijk, Klaas
Bentolila, Stephane
Hanson, Maureen R.
A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing
title A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing
title_full A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing
title_fullStr A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing
title_full_unstemmed A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing
title_short A Zinc Finger Motif-Containing Protein Is Essential for Chloroplast RNA Editing
title_sort zinc finger motif-containing protein is essential for chloroplast rna editing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359148/
https://www.ncbi.nlm.nih.gov/pubmed/25768119
http://dx.doi.org/10.1371/journal.pgen.1005028
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