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PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma

Pyruvate kinase M2 (PKM2) is a member of the pyruvate kinase family. Recent work has defined the “non-metabolic” functions of PKM2. However, the role of PKM2 in HCC remains unclear. To investigate the role of PKM2 in tumor growth, invasion and the prognosis of hepatocellular carcinoma (HCC), PKM2 ex...

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Autores principales: Liu, Wei-Ren, Tian, Meng-Xin, Yang, Liu-Xiao, Lin, Yu-Li, Jin, Lei, Ding, Zhen-Bin, Shen, Ying-Hao, Peng, Yuan-Fei, Gao, Dong-Mei, Zhou, Jian, Qiu, Shuang-Jian, Dai, Zhi, He, Rui, Fan, Jia, Shi, Ying-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359260/
https://www.ncbi.nlm.nih.gov/pubmed/25514599
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author Liu, Wei-Ren
Tian, Meng-Xin
Yang, Liu-Xiao
Lin, Yu-Li
Jin, Lei
Ding, Zhen-Bin
Shen, Ying-Hao
Peng, Yuan-Fei
Gao, Dong-Mei
Zhou, Jian
Qiu, Shuang-Jian
Dai, Zhi
He, Rui
Fan, Jia
Shi, Ying-Hong
author_facet Liu, Wei-Ren
Tian, Meng-Xin
Yang, Liu-Xiao
Lin, Yu-Li
Jin, Lei
Ding, Zhen-Bin
Shen, Ying-Hao
Peng, Yuan-Fei
Gao, Dong-Mei
Zhou, Jian
Qiu, Shuang-Jian
Dai, Zhi
He, Rui
Fan, Jia
Shi, Ying-Hong
author_sort Liu, Wei-Ren
collection PubMed
description Pyruvate kinase M2 (PKM2) is a member of the pyruvate kinase family. Recent work has defined the “non-metabolic” functions of PKM2. However, the role of PKM2 in HCC remains unclear. To investigate the role of PKM2 in tumor growth, invasion and the prognosis of hepatocellular carcinoma (HCC), PKM2 expression was measured in HCC cell lines and tissues using qRT-PCR, western blot, and immunofluorescence assays. In in vitro experiments, PKM2 was knocked down using a short hairpin RNA lentivirus vector, and tumor cell behavior and the downstream signaling pathways and chemokine were analyzed. For the analysis of in vivo tumor growth, intratumoral and peritumoral lymphocyte infiltration were examined in nude mice. The prognostic value of PKM2 was analyzed by immunohistochemistry in two cohorts including 721 HCC patients. Together, our data obtained from cell lines, tumorigenicity studies, and primary HCC samples illustrate an oncogenic role for PKM2 in tumors. Moreover, PKM2 may serve as a novel prognostic indicator for HCC patients after curative resection, targeted therapy aimed at PKM2 may represent an effective treatment approach for HCC.
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spelling pubmed-43592602015-03-27 PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma Liu, Wei-Ren Tian, Meng-Xin Yang, Liu-Xiao Lin, Yu-Li Jin, Lei Ding, Zhen-Bin Shen, Ying-Hao Peng, Yuan-Fei Gao, Dong-Mei Zhou, Jian Qiu, Shuang-Jian Dai, Zhi He, Rui Fan, Jia Shi, Ying-Hong Oncotarget Research Paper Pyruvate kinase M2 (PKM2) is a member of the pyruvate kinase family. Recent work has defined the “non-metabolic” functions of PKM2. However, the role of PKM2 in HCC remains unclear. To investigate the role of PKM2 in tumor growth, invasion and the prognosis of hepatocellular carcinoma (HCC), PKM2 expression was measured in HCC cell lines and tissues using qRT-PCR, western blot, and immunofluorescence assays. In in vitro experiments, PKM2 was knocked down using a short hairpin RNA lentivirus vector, and tumor cell behavior and the downstream signaling pathways and chemokine were analyzed. For the analysis of in vivo tumor growth, intratumoral and peritumoral lymphocyte infiltration were examined in nude mice. The prognostic value of PKM2 was analyzed by immunohistochemistry in two cohorts including 721 HCC patients. Together, our data obtained from cell lines, tumorigenicity studies, and primary HCC samples illustrate an oncogenic role for PKM2 in tumors. Moreover, PKM2 may serve as a novel prognostic indicator for HCC patients after curative resection, targeted therapy aimed at PKM2 may represent an effective treatment approach for HCC. Impact Journals LLC 2014-12-02 /pmc/articles/PMC4359260/ /pubmed/25514599 Text en Copyright: © 2015 Liu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Wei-Ren
Tian, Meng-Xin
Yang, Liu-Xiao
Lin, Yu-Li
Jin, Lei
Ding, Zhen-Bin
Shen, Ying-Hao
Peng, Yuan-Fei
Gao, Dong-Mei
Zhou, Jian
Qiu, Shuang-Jian
Dai, Zhi
He, Rui
Fan, Jia
Shi, Ying-Hong
PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
title PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
title_full PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
title_fullStr PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
title_full_unstemmed PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
title_short PKM2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
title_sort pkm2 promotes metastasis by recruiting myeloid-derived suppressor cells and indicates poor prognosis for hepatocellular carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359260/
https://www.ncbi.nlm.nih.gov/pubmed/25514599
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