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Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359305/ https://www.ncbi.nlm.nih.gov/pubmed/25544762 |
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author | Giorgi, Carlotta Bonora, Massimo Missiroli, Sonia Poletti, Federica Ramirez, Fabian Galindo Morciano, Giampaolo Morganti, Claudia Pandolfi, Pier Paolo Mammano, Fabio Pinton, Paolo |
author_facet | Giorgi, Carlotta Bonora, Massimo Missiroli, Sonia Poletti, Federica Ramirez, Fabian Galindo Morciano, Giampaolo Morganti, Claudia Pandolfi, Pier Paolo Mammano, Fabio Pinton, Paolo |
author_sort | Giorgi, Carlotta |
collection | PubMed |
description | One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate the second messenger calcium (Ca(2+)). In the present study, we established conditions that allow the in vivo detection of Ca(2+) signaling in three-dimensional tumor masses in mouse models. By combining intravital imaging and a skinfold chamber technique, we determined the ability of photodynamic cancer therapy to induce an increase in intracellular Ca(2+) concentrations and, consequently, an increase in cell death in a p53-dependent pathway. |
format | Online Article Text |
id | pubmed-4359305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-43593052015-03-26 Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling Giorgi, Carlotta Bonora, Massimo Missiroli, Sonia Poletti, Federica Ramirez, Fabian Galindo Morciano, Giampaolo Morganti, Claudia Pandolfi, Pier Paolo Mammano, Fabio Pinton, Paolo Oncotarget Research Paper One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate the second messenger calcium (Ca(2+)). In the present study, we established conditions that allow the in vivo detection of Ca(2+) signaling in three-dimensional tumor masses in mouse models. By combining intravital imaging and a skinfold chamber technique, we determined the ability of photodynamic cancer therapy to induce an increase in intracellular Ca(2+) concentrations and, consequently, an increase in cell death in a p53-dependent pathway. Impact Journals LLC 2014-12-02 /pmc/articles/PMC4359305/ /pubmed/25544762 Text en Copyright: © 2015 Giorgi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Giorgi, Carlotta Bonora, Massimo Missiroli, Sonia Poletti, Federica Ramirez, Fabian Galindo Morciano, Giampaolo Morganti, Claudia Pandolfi, Pier Paolo Mammano, Fabio Pinton, Paolo Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
title | Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
title_full | Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
title_fullStr | Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
title_full_unstemmed | Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
title_short | Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
title_sort | intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359305/ https://www.ncbi.nlm.nih.gov/pubmed/25544762 |
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