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Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling

One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate...

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Autores principales: Giorgi, Carlotta, Bonora, Massimo, Missiroli, Sonia, Poletti, Federica, Ramirez, Fabian Galindo, Morciano, Giampaolo, Morganti, Claudia, Pandolfi, Pier Paolo, Mammano, Fabio, Pinton, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359305/
https://www.ncbi.nlm.nih.gov/pubmed/25544762
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author Giorgi, Carlotta
Bonora, Massimo
Missiroli, Sonia
Poletti, Federica
Ramirez, Fabian Galindo
Morciano, Giampaolo
Morganti, Claudia
Pandolfi, Pier Paolo
Mammano, Fabio
Pinton, Paolo
author_facet Giorgi, Carlotta
Bonora, Massimo
Missiroli, Sonia
Poletti, Federica
Ramirez, Fabian Galindo
Morciano, Giampaolo
Morganti, Claudia
Pandolfi, Pier Paolo
Mammano, Fabio
Pinton, Paolo
author_sort Giorgi, Carlotta
collection PubMed
description One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate the second messenger calcium (Ca(2+)). In the present study, we established conditions that allow the in vivo detection of Ca(2+) signaling in three-dimensional tumor masses in mouse models. By combining intravital imaging and a skinfold chamber technique, we determined the ability of photodynamic cancer therapy to induce an increase in intracellular Ca(2+) concentrations and, consequently, an increase in cell death in a p53-dependent pathway.
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spelling pubmed-43593052015-03-26 Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling Giorgi, Carlotta Bonora, Massimo Missiroli, Sonia Poletti, Federica Ramirez, Fabian Galindo Morciano, Giampaolo Morganti, Claudia Pandolfi, Pier Paolo Mammano, Fabio Pinton, Paolo Oncotarget Research Paper One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate the second messenger calcium (Ca(2+)). In the present study, we established conditions that allow the in vivo detection of Ca(2+) signaling in three-dimensional tumor masses in mouse models. By combining intravital imaging and a skinfold chamber technique, we determined the ability of photodynamic cancer therapy to induce an increase in intracellular Ca(2+) concentrations and, consequently, an increase in cell death in a p53-dependent pathway. Impact Journals LLC 2014-12-02 /pmc/articles/PMC4359305/ /pubmed/25544762 Text en Copyright: © 2015 Giorgi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Giorgi, Carlotta
Bonora, Massimo
Missiroli, Sonia
Poletti, Federica
Ramirez, Fabian Galindo
Morciano, Giampaolo
Morganti, Claudia
Pandolfi, Pier Paolo
Mammano, Fabio
Pinton, Paolo
Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
title Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
title_full Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
title_fullStr Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
title_full_unstemmed Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
title_short Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
title_sort intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359305/
https://www.ncbi.nlm.nih.gov/pubmed/25544762
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