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A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data
Aging is characterized by a profound remodeling of the epigenetic architecture in terms of DNA methylation patterns. To date the most effective tool to study genome wide DNA methylation changes is Infinium HumanMethylation450 BeadChip (Infinium 450k). Despite the wealth of tools for Infinium 450k an...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359692/ https://www.ncbi.nlm.nih.gov/pubmed/25701668 |
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author | Bacalini, Maria Giulia Boattini, Alessio Gentilini, Davide Giampieri, Enrico Pirazzini, Chiara Giuliani, Cristina Fontanesi, Elisa Remondini, Daniel Capri, Miriam Del Rio, Alberto Luiselli, Donata Vitale, Giovanni Mari, Daniela Castellani, Gastone Di Blasio, Anna Maria Salvioli, Stefano Franceschi, Claudio Garagnani, Paolo |
author_facet | Bacalini, Maria Giulia Boattini, Alessio Gentilini, Davide Giampieri, Enrico Pirazzini, Chiara Giuliani, Cristina Fontanesi, Elisa Remondini, Daniel Capri, Miriam Del Rio, Alberto Luiselli, Donata Vitale, Giovanni Mari, Daniela Castellani, Gastone Di Blasio, Anna Maria Salvioli, Stefano Franceschi, Claudio Garagnani, Paolo |
author_sort | Bacalini, Maria Giulia |
collection | PubMed |
description | Aging is characterized by a profound remodeling of the epigenetic architecture in terms of DNA methylation patterns. To date the most effective tool to study genome wide DNA methylation changes is Infinium HumanMethylation450 BeadChip (Infinium 450k). Despite the wealth of tools for Infinium 450k analysis, the identification of the most biologically relevant DNA methylation changes is still challenging. Here we propose an analytical pipeline to select differentially methylated regions (DMRs), tailored on microarray architecture, which is highly effective in highlighting biologically relevant results. The pipeline groups microarray probes on the basis of their localization respect to CpG islands and genic sequences and, depending on probes density, identifies DMRs through a single-probe or a region-centric approach that considers the concomitant variation of multiple adjacent CpG probes. We successfully applied this analytical pipeline on 3 independent Infinium 450k datasets that investigated age-associated changes in blood DNA methylation. We provide a consensus list of genes that systematically vary in DNA methylation levels from 0 to 100 years and that have a potentially relevant role in the aging process. |
format | Online Article Text |
id | pubmed-4359692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-43596922015-03-27 A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data Bacalini, Maria Giulia Boattini, Alessio Gentilini, Davide Giampieri, Enrico Pirazzini, Chiara Giuliani, Cristina Fontanesi, Elisa Remondini, Daniel Capri, Miriam Del Rio, Alberto Luiselli, Donata Vitale, Giovanni Mari, Daniela Castellani, Gastone Di Blasio, Anna Maria Salvioli, Stefano Franceschi, Claudio Garagnani, Paolo Aging (Albany NY) Research Paper Aging is characterized by a profound remodeling of the epigenetic architecture in terms of DNA methylation patterns. To date the most effective tool to study genome wide DNA methylation changes is Infinium HumanMethylation450 BeadChip (Infinium 450k). Despite the wealth of tools for Infinium 450k analysis, the identification of the most biologically relevant DNA methylation changes is still challenging. Here we propose an analytical pipeline to select differentially methylated regions (DMRs), tailored on microarray architecture, which is highly effective in highlighting biologically relevant results. The pipeline groups microarray probes on the basis of their localization respect to CpG islands and genic sequences and, depending on probes density, identifies DMRs through a single-probe or a region-centric approach that considers the concomitant variation of multiple adjacent CpG probes. We successfully applied this analytical pipeline on 3 independent Infinium 450k datasets that investigated age-associated changes in blood DNA methylation. We provide a consensus list of genes that systematically vary in DNA methylation levels from 0 to 100 years and that have a potentially relevant role in the aging process. Impact Journals LLC 2015-01-11 /pmc/articles/PMC4359692/ /pubmed/25701668 Text en Copyright: © 2015 Bacalini et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Bacalini, Maria Giulia Boattini, Alessio Gentilini, Davide Giampieri, Enrico Pirazzini, Chiara Giuliani, Cristina Fontanesi, Elisa Remondini, Daniel Capri, Miriam Del Rio, Alberto Luiselli, Donata Vitale, Giovanni Mari, Daniela Castellani, Gastone Di Blasio, Anna Maria Salvioli, Stefano Franceschi, Claudio Garagnani, Paolo A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data |
title | A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data |
title_full | A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data |
title_fullStr | A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data |
title_full_unstemmed | A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data |
title_short | A meta-analysis on age-associated changes in blood DNA methylation: results from an original analysis pipeline for Infinium 450k data |
title_sort | meta-analysis on age-associated changes in blood dna methylation: results from an original analysis pipeline for infinium 450k data |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359692/ https://www.ncbi.nlm.nih.gov/pubmed/25701668 |
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