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Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene

Sugarcane is one of the most important crops cultivated for the production of sugar and ethanol. In our previous studies, an innovative positive selection system for obtaining transgenic sugarcane, which utilized the E. coli-derived manA gene as the selectable marker and mannose as the selective age...

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Autores principales: Zhang, Muqing, Zhuo, Xiaolei, Wang, Jihua, Yang, Chuanyu, Powell, Charles A., Chen, Rukai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359708/
https://www.ncbi.nlm.nih.gov/pubmed/25798049
http://dx.doi.org/10.1007/s11032-015-0295-4
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author Zhang, Muqing
Zhuo, Xiaolei
Wang, Jihua
Yang, Chuanyu
Powell, Charles A.
Chen, Rukai
author_facet Zhang, Muqing
Zhuo, Xiaolei
Wang, Jihua
Yang, Chuanyu
Powell, Charles A.
Chen, Rukai
author_sort Zhang, Muqing
collection PubMed
description Sugarcane is one of the most important crops cultivated for the production of sugar and ethanol. In our previous studies, an innovative positive selection system for obtaining transgenic sugarcane, which utilized the E. coli-derived manA gene as the selectable marker and mannose as the selective agent, was developed and patented in China. In this paper, the influence of phosphomannose isomerase (PMI) overexpression on the key enzymes of both glycolysis and sucrose metabolism was investigated in transgenic sugarcane through the manA gene. Overexpressed PMI increased hexokinase activity by approximately 24 % compared with non-transgenic control plants, but pyruvate kinase (PK) activity was reduced by approximately 14 %. In comparison with the non-transgenic control plants, the activities of sucrose synthase, sucrose-phosphate synthase, and acid invertase were also modestly affected in the PMI-overexpressing transgenic plants, but no significant differences were observed at the stalk elongation and maturity stages. However, agronomic and technical traits were not affected by manA gene overexpression in the transgenic sugarcane. In conclusion, PMI overexpression significantly affected the hexokinase and PK activities by catalyzing the reversible interconversion between mannose-6-phosphate and fructose-6-phosphate, which is an intermediate of glycolysis. However, it had no significant effects on sucrose accumulation in sugarcane. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-015-0295-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-43597082015-03-18 Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene Zhang, Muqing Zhuo, Xiaolei Wang, Jihua Yang, Chuanyu Powell, Charles A. Chen, Rukai Mol Breed Article Sugarcane is one of the most important crops cultivated for the production of sugar and ethanol. In our previous studies, an innovative positive selection system for obtaining transgenic sugarcane, which utilized the E. coli-derived manA gene as the selectable marker and mannose as the selective agent, was developed and patented in China. In this paper, the influence of phosphomannose isomerase (PMI) overexpression on the key enzymes of both glycolysis and sucrose metabolism was investigated in transgenic sugarcane through the manA gene. Overexpressed PMI increased hexokinase activity by approximately 24 % compared with non-transgenic control plants, but pyruvate kinase (PK) activity was reduced by approximately 14 %. In comparison with the non-transgenic control plants, the activities of sucrose synthase, sucrose-phosphate synthase, and acid invertase were also modestly affected in the PMI-overexpressing transgenic plants, but no significant differences were observed at the stalk elongation and maturity stages. However, agronomic and technical traits were not affected by manA gene overexpression in the transgenic sugarcane. In conclusion, PMI overexpression significantly affected the hexokinase and PK activities by catalyzing the reversible interconversion between mannose-6-phosphate and fructose-6-phosphate, which is an intermediate of glycolysis. However, it had no significant effects on sucrose accumulation in sugarcane. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-015-0295-4) contains supplementary material, which is available to authorized users. Springer Netherlands 2015-03-15 2015 /pmc/articles/PMC4359708/ /pubmed/25798049 http://dx.doi.org/10.1007/s11032-015-0295-4 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Zhang, Muqing
Zhuo, Xiaolei
Wang, Jihua
Yang, Chuanyu
Powell, Charles A.
Chen, Rukai
Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene
title Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene
title_full Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene
title_fullStr Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene
title_full_unstemmed Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene
title_short Phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the manA gene
title_sort phosphomannose isomerase affects the key enzymes of glycolysis and sucrose metabolism in transgenic sugarcane overexpressing the mana gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359708/
https://www.ncbi.nlm.nih.gov/pubmed/25798049
http://dx.doi.org/10.1007/s11032-015-0295-4
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