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Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas

Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in r...

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Autores principales: Rajnai, Hajnalka, Teleki, Ivett, Kiszner, Gergo, Meggyesházi, Nora, Balla, Peter, Vancsik, Tamas, Muzes, Gyorgyi, Csomor, Judit, Matolcsy, Andras, Krenacs, Tibor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359865/
https://www.ncbi.nlm.nih.gov/pubmed/25815348
http://dx.doi.org/10.1155/2015/528098
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author Rajnai, Hajnalka
Teleki, Ivett
Kiszner, Gergo
Meggyesházi, Nora
Balla, Peter
Vancsik, Tamas
Muzes, Gyorgyi
Csomor, Judit
Matolcsy, Andras
Krenacs, Tibor
author_facet Rajnai, Hajnalka
Teleki, Ivett
Kiszner, Gergo
Meggyesházi, Nora
Balla, Peter
Vancsik, Tamas
Muzes, Gyorgyi
Csomor, Judit
Matolcsy, Andras
Krenacs, Tibor
author_sort Rajnai, Hajnalka
collection PubMed
description Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell clusters (resembling to “ex vivo” germinal centers) with Gap27 peptide, mimicking the 2nd extracellular loop of Cx43 protein, significantly impaired FDC-B cell cluster formation and cell survival. In untreated cultures of intact clusters, cell proliferation showed a moderate reduction. In tissues, Cx43 protein levels run parallel with the density of FDC both in reactive germinal centers and in malformed follicles of follicular lymphomas and showed strong upregulation in newly generated and/or degrading bi-/multinuclear FDC of rudimentary processes. However, the inverse correlation between Cx43 expression and B cell proliferation seen in reactive germinal centers was not detected in follicular lymphomas. Furthermore, Cx43 levels were not associated with either lymphoma grade or bone marrow involvement. Our results suggest that Cx43 channels are critical in FDC and “ex vivo” germinal center development and in the persistence of FDC in follicular lymphomas but do not affect tumor progression.
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spelling pubmed-43598652015-03-26 Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas Rajnai, Hajnalka Teleki, Ivett Kiszner, Gergo Meggyesházi, Nora Balla, Peter Vancsik, Tamas Muzes, Gyorgyi Csomor, Judit Matolcsy, Andras Krenacs, Tibor J Immunol Res Research Article Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell clusters (resembling to “ex vivo” germinal centers) with Gap27 peptide, mimicking the 2nd extracellular loop of Cx43 protein, significantly impaired FDC-B cell cluster formation and cell survival. In untreated cultures of intact clusters, cell proliferation showed a moderate reduction. In tissues, Cx43 protein levels run parallel with the density of FDC both in reactive germinal centers and in malformed follicles of follicular lymphomas and showed strong upregulation in newly generated and/or degrading bi-/multinuclear FDC of rudimentary processes. However, the inverse correlation between Cx43 expression and B cell proliferation seen in reactive germinal centers was not detected in follicular lymphomas. Furthermore, Cx43 levels were not associated with either lymphoma grade or bone marrow involvement. Our results suggest that Cx43 channels are critical in FDC and “ex vivo” germinal center development and in the persistence of FDC in follicular lymphomas but do not affect tumor progression. Hindawi Publishing Corporation 2015 2015-03-02 /pmc/articles/PMC4359865/ /pubmed/25815348 http://dx.doi.org/10.1155/2015/528098 Text en Copyright © 2015 Hajnalka Rajnai et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rajnai, Hajnalka
Teleki, Ivett
Kiszner, Gergo
Meggyesházi, Nora
Balla, Peter
Vancsik, Tamas
Muzes, Gyorgyi
Csomor, Judit
Matolcsy, Andras
Krenacs, Tibor
Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
title Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
title_full Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
title_fullStr Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
title_full_unstemmed Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
title_short Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
title_sort connexin 43 communication channels in follicular dendritic cell development and in follicular lymphomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359865/
https://www.ncbi.nlm.nih.gov/pubmed/25815348
http://dx.doi.org/10.1155/2015/528098
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