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Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis

OBJECTIVE: Cytotoxic T lymphocyte antigen-4 (CTLA-4) is one of the basic antigens involved in immune responses regulation associated with autoimmune thyroid diseases. The aim of the study was to evaluate whether the surface expression of CTLA-4(CD152) on T cells is correlated with laboratory autoimm...

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Autores principales: Kucharska, AM, Gorska, E, Wasik, M, Pyrzak, B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4360358/
https://www.ncbi.nlm.nih.gov/pubmed/21147625
http://dx.doi.org/10.1186/2047-783X-15-S2-72
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author Kucharska, AM
Gorska, E
Wasik, M
Pyrzak, B
author_facet Kucharska, AM
Gorska, E
Wasik, M
Pyrzak, B
author_sort Kucharska, AM
collection PubMed
description OBJECTIVE: Cytotoxic T lymphocyte antigen-4 (CTLA-4) is one of the basic antigens involved in immune responses regulation associated with autoimmune thyroid diseases. The aim of the study was to evaluate whether the surface expression of CTLA-4(CD152) on T cells is correlated with laboratory autoimmune markers in children with Hashimoto's disease. MATERIAL AND METHODS: Blood samples were obtained from 45 children with Hashimoto's thyroiditis of the mean age 14.8 ± 2.35 years, and from 55 healthy age-matched children, free of allergic, immune and hematological disorders, and with a normal thyroid function. The anti-thyroid antibodies were measured with Microparticle Enzyme Immunoassay (AxSYM Anti-Tg, AxSYM Anti-TPO). The T cell phenotype was evaluated flow cytometery, with the use of monoclonal antibodies combination: CD4- FITC/CD28 -PC5/CD152 -PE and CD8 -FITC/CD28 -PC5/CD152 -PE RESULTS: The percentage of T cells with CD152 expression was significantly decreased in children with Hashimoto's thyroiditis compared with healthy controls (P < 0.001). A significant negative correlation was found between the level of anti-thyroglobulin antibodies and the percentage of CD4+CD152+ T cells (r = -0.34; P < 0.05). Anti-thyroperoxidase antibodies did not correlate with CD152 expression. CONCLUSIONS: In children with Hashimoto's thyroiditis, the number of CD4+CD152+ T cells is decreased and negatively correlates with the level of anti-thyroglobulin antibodies.
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spelling pubmed-43603582015-03-26 Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis Kucharska, AM Gorska, E Wasik, M Pyrzak, B Eur J Med Res Research OBJECTIVE: Cytotoxic T lymphocyte antigen-4 (CTLA-4) is one of the basic antigens involved in immune responses regulation associated with autoimmune thyroid diseases. The aim of the study was to evaluate whether the surface expression of CTLA-4(CD152) on T cells is correlated with laboratory autoimmune markers in children with Hashimoto's disease. MATERIAL AND METHODS: Blood samples were obtained from 45 children with Hashimoto's thyroiditis of the mean age 14.8 ± 2.35 years, and from 55 healthy age-matched children, free of allergic, immune and hematological disorders, and with a normal thyroid function. The anti-thyroid antibodies were measured with Microparticle Enzyme Immunoassay (AxSYM Anti-Tg, AxSYM Anti-TPO). The T cell phenotype was evaluated flow cytometery, with the use of monoclonal antibodies combination: CD4- FITC/CD28 -PC5/CD152 -PE and CD8 -FITC/CD28 -PC5/CD152 -PE RESULTS: The percentage of T cells with CD152 expression was significantly decreased in children with Hashimoto's thyroiditis compared with healthy controls (P < 0.001). A significant negative correlation was found between the level of anti-thyroglobulin antibodies and the percentage of CD4+CD152+ T cells (r = -0.34; P < 0.05). Anti-thyroperoxidase antibodies did not correlate with CD152 expression. CONCLUSIONS: In children with Hashimoto's thyroiditis, the number of CD4+CD152+ T cells is decreased and negatively correlates with the level of anti-thyroglobulin antibodies. BioMed Central 2010-11-04 /pmc/articles/PMC4360358/ /pubmed/21147625 http://dx.doi.org/10.1186/2047-783X-15-S2-72 Text en Copyright © 2010 I. Holzapfel Publishers
spellingShingle Research
Kucharska, AM
Gorska, E
Wasik, M
Pyrzak, B
Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
title Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
title_full Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
title_fullStr Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
title_full_unstemmed Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
title_short Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
title_sort decreased cd4+cd152+ t cell subset and its correlation with the level of antithyroid antibodies in children with chronic autoimmune thyroiditis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4360358/
https://www.ncbi.nlm.nih.gov/pubmed/21147625
http://dx.doi.org/10.1186/2047-783X-15-S2-72
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