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SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones
BACKGROUND: cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high percentage of 5'-truncated clones. Current library construction methods that enrich for full-length mRNA ar...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC436056/ https://www.ncbi.nlm.nih.gov/pubmed/15198809 http://dx.doi.org/10.1186/1471-2164-5-36 |