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Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series

Phylogenetic analysis of partial mitochondrial cytochrome oxidase c subunit I (COI) and nuclear internal transcribed spacer 2 (ITS2) sequences were used to evaluate initial identification and to investigate phylogenetic relationships of seven Anopheles morphospecies of the Arribalzagia Series from C...

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Autores principales: Gómez, Giovan F., Bickersmith, Sara A., González, Ranulfo, Conn, Jan E., Correa, Margarita M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361172/
https://www.ncbi.nlm.nih.gov/pubmed/25774795
http://dx.doi.org/10.1371/journal.pone.0119488
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author Gómez, Giovan F.
Bickersmith, Sara A.
González, Ranulfo
Conn, Jan E.
Correa, Margarita M.
author_facet Gómez, Giovan F.
Bickersmith, Sara A.
González, Ranulfo
Conn, Jan E.
Correa, Margarita M.
author_sort Gómez, Giovan F.
collection PubMed
description Phylogenetic analysis of partial mitochondrial cytochrome oxidase c subunit I (COI) and nuclear internal transcribed spacer 2 (ITS2) sequences were used to evaluate initial identification and to investigate phylogenetic relationships of seven Anopheles morphospecies of the Arribalzagia Series from Colombia. Phylogenetic trees recovered highly supported clades for An. punctimaculas.s., An. calderoni, An. malefactor s.l., An. neomaculipalpus, An. apicimacula s.l., An. mattogrossensis and An. peryassui. This study provides the first molecular confirmation of An. malefactorfrom Colombia and discovered conflicting patterns of divergence for the molecular markers among specimens from northeast and northern Colombia suggesting the presence of two previously unrecognized Molecular Operational Taxonomic Units (MOTUs). Furthermore, two highly differentiated An. apicimacula MOTUs previously found in Panama were detected. Overall, the combined molecular dataset facilitated the detection of known and new Colombian evolutionary lineages, and constitutes the baseline for future research on their bionomics, ecology and potential role as malaria vectors.
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spelling pubmed-43611722015-03-23 Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series Gómez, Giovan F. Bickersmith, Sara A. González, Ranulfo Conn, Jan E. Correa, Margarita M. PLoS One Research Article Phylogenetic analysis of partial mitochondrial cytochrome oxidase c subunit I (COI) and nuclear internal transcribed spacer 2 (ITS2) sequences were used to evaluate initial identification and to investigate phylogenetic relationships of seven Anopheles morphospecies of the Arribalzagia Series from Colombia. Phylogenetic trees recovered highly supported clades for An. punctimaculas.s., An. calderoni, An. malefactor s.l., An. neomaculipalpus, An. apicimacula s.l., An. mattogrossensis and An. peryassui. This study provides the first molecular confirmation of An. malefactorfrom Colombia and discovered conflicting patterns of divergence for the molecular markers among specimens from northeast and northern Colombia suggesting the presence of two previously unrecognized Molecular Operational Taxonomic Units (MOTUs). Furthermore, two highly differentiated An. apicimacula MOTUs previously found in Panama were detected. Overall, the combined molecular dataset facilitated the detection of known and new Colombian evolutionary lineages, and constitutes the baseline for future research on their bionomics, ecology and potential role as malaria vectors. Public Library of Science 2015-03-16 /pmc/articles/PMC4361172/ /pubmed/25774795 http://dx.doi.org/10.1371/journal.pone.0119488 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Gómez, Giovan F.
Bickersmith, Sara A.
González, Ranulfo
Conn, Jan E.
Correa, Margarita M.
Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series
title Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series
title_full Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series
title_fullStr Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series
title_full_unstemmed Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series
title_short Molecular Taxonomy Provides New Insights into Anopheles Species of the Neotropical Arribalzagia Series
title_sort molecular taxonomy provides new insights into anopheles species of the neotropical arribalzagia series
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361172/
https://www.ncbi.nlm.nih.gov/pubmed/25774795
http://dx.doi.org/10.1371/journal.pone.0119488
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