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Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction

Intracranial pressure and volume vary considerably between hydrocephalic patients, and with age, health and haemodynamic status; if left untreated, intracranial pressure rises and the ventricular system expands to accommodate the excess cerebrospinal fluid, with significant morbidity and mortality....

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Autores principales: Suresh, Supraja, Black, Richard A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361491/
https://www.ncbi.nlm.nih.gov/pubmed/25245779
http://dx.doi.org/10.1177/0885328214551587
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author Suresh, Supraja
Black, Richard A
author_facet Suresh, Supraja
Black, Richard A
author_sort Suresh, Supraja
collection PubMed
description Intracranial pressure and volume vary considerably between hydrocephalic patients, and with age, health and haemodynamic status; if left untreated, intracranial pressure rises and the ventricular system expands to accommodate the excess cerebrospinal fluid, with significant morbidity and mortality. Cerebrospinal fluid shunts in use today have a high incidence of failure with shunt obstruction being the most serious. Conventional proximal shunt catheters are made from poly(dimethyl)siloxane, the walls of which are perforated with holes for the cerebrospinal fluid to pass through. The limited range of catheters, in terms of material selection and flow distribution, is responsible in large part for their poor performance. In this study, we present an alternative design of proximal catheter made of electrospun polyether urethane, and evaluate its performance in the presence of glial cells, which are responsible for shunt blockage. The viability and growth of cells on catheter materials such as poly(dimethyl)siloxane and polyurethane in the form of cast films, microfibrous mats and porous sponges were studied in the presence of proteins present in cerebrospinal fluid after 48 h and 96 h in culture. The numbers of viable cells on each substrate were comparable to untreated poly(dimethyl)siloxane, both in the presence and absence of serum proteins found in cerebrospinal fluid. A cell culture model of shunt obstruction was developed in which cells on electrospun polyether urethane catheters were subjected to flow during culture in vitro, and the degree of obstruction quantified in terms of hydraulic permeability after static and perfusion culture. The results indicate that a catheter made of electrospun polyether urethane would be able to maintain cerebrospinal fluid flow even with the presence of cells for the time period chosen for this study. These findings have implications for the design and deployment of microporous shunt catheter systems for the treatment of hydrocephalus.
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spelling pubmed-43614912015-04-10 Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction Suresh, Supraja Black, Richard A J Biomater Appl Nanotechnology in Biomaterials Intracranial pressure and volume vary considerably between hydrocephalic patients, and with age, health and haemodynamic status; if left untreated, intracranial pressure rises and the ventricular system expands to accommodate the excess cerebrospinal fluid, with significant morbidity and mortality. Cerebrospinal fluid shunts in use today have a high incidence of failure with shunt obstruction being the most serious. Conventional proximal shunt catheters are made from poly(dimethyl)siloxane, the walls of which are perforated with holes for the cerebrospinal fluid to pass through. The limited range of catheters, in terms of material selection and flow distribution, is responsible in large part for their poor performance. In this study, we present an alternative design of proximal catheter made of electrospun polyether urethane, and evaluate its performance in the presence of glial cells, which are responsible for shunt blockage. The viability and growth of cells on catheter materials such as poly(dimethyl)siloxane and polyurethane in the form of cast films, microfibrous mats and porous sponges were studied in the presence of proteins present in cerebrospinal fluid after 48 h and 96 h in culture. The numbers of viable cells on each substrate were comparable to untreated poly(dimethyl)siloxane, both in the presence and absence of serum proteins found in cerebrospinal fluid. A cell culture model of shunt obstruction was developed in which cells on electrospun polyether urethane catheters were subjected to flow during culture in vitro, and the degree of obstruction quantified in terms of hydraulic permeability after static and perfusion culture. The results indicate that a catheter made of electrospun polyether urethane would be able to maintain cerebrospinal fluid flow even with the presence of cells for the time period chosen for this study. These findings have implications for the design and deployment of microporous shunt catheter systems for the treatment of hydrocephalus. SAGE Publications 2015-02 /pmc/articles/PMC4361491/ /pubmed/25245779 http://dx.doi.org/10.1177/0885328214551587 Text en © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(http://www.uk.sagepub.com/aboutus/openaccess.htm).
spellingShingle Nanotechnology in Biomaterials
Suresh, Supraja
Black, Richard A
Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
title Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
title_full Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
title_fullStr Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
title_full_unstemmed Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
title_short Electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
title_sort electrospun polyurethane as an alternative ventricular catheter and in vitro model of shunt obstruction
topic Nanotechnology in Biomaterials
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361491/
https://www.ncbi.nlm.nih.gov/pubmed/25245779
http://dx.doi.org/10.1177/0885328214551587
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