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SYBR Green Real-Time PCR-RFLP Assay Targeting the Plasmodium Cytochrome B Gene – A Highly Sensitive Molecular Tool for Malaria Parasite Detection and Species Determination

A prerequisite for reliable detection of low-density Plasmodium infections in malaria pre-elimination settings is the availability of ultra-sensitive and high-throughput molecular tools. We developed a SYBR Green real-time PCR restriction fragment length polymorphism assay (cytb-qPCR) targeting the...

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Detalles Bibliográficos
Autores principales: Xu, Weiping, Morris, Ulrika, Aydin-Schmidt, Berit, Msellem, Mwinyi I., Shakely, Delér, Petzold, Max, Björkman, Anders, Mårtensson, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361616/
https://www.ncbi.nlm.nih.gov/pubmed/25774805
http://dx.doi.org/10.1371/journal.pone.0120210