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Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus

We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the pre...

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Autores principales: Canduela, Miren-Josune, Mendizabal-Zubiaga, Juan, Puente, Nagore, Reguero, Leire, Elezgarai, Izaskun, Ramos-Uriarte, Almudena, Gerrikagoitia, Inmaculada, Grandes, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361705/
https://www.ncbi.nlm.nih.gov/pubmed/25775089
http://dx.doi.org/10.1371/journal.pone.0119401
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author Canduela, Miren-Josune
Mendizabal-Zubiaga, Juan
Puente, Nagore
Reguero, Leire
Elezgarai, Izaskun
Ramos-Uriarte, Almudena
Gerrikagoitia, Inmaculada
Grandes, Pedro
author_facet Canduela, Miren-Josune
Mendizabal-Zubiaga, Juan
Puente, Nagore
Reguero, Leire
Elezgarai, Izaskun
Ramos-Uriarte, Almudena
Gerrikagoitia, Inmaculada
Grandes, Pedro
author_sort Canduela, Miren-Josune
collection PubMed
description We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (∼60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present.
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spelling pubmed-43617052015-03-23 Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus Canduela, Miren-Josune Mendizabal-Zubiaga, Juan Puente, Nagore Reguero, Leire Elezgarai, Izaskun Ramos-Uriarte, Almudena Gerrikagoitia, Inmaculada Grandes, Pedro PLoS One Research Article We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (∼60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present. Public Library of Science 2015-03-16 /pmc/articles/PMC4361705/ /pubmed/25775089 http://dx.doi.org/10.1371/journal.pone.0119401 Text en © 2015 Canduela et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Canduela, Miren-Josune
Mendizabal-Zubiaga, Juan
Puente, Nagore
Reguero, Leire
Elezgarai, Izaskun
Ramos-Uriarte, Almudena
Gerrikagoitia, Inmaculada
Grandes, Pedro
Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
title Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
title_full Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
title_fullStr Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
title_full_unstemmed Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
title_short Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
title_sort visualization by high resolution immunoelectron microscopy of the transient receptor potential vanilloid-1 at inhibitory synapses of the mouse dentate gyrus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361705/
https://www.ncbi.nlm.nih.gov/pubmed/25775089
http://dx.doi.org/10.1371/journal.pone.0119401
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