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Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus
We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the pre...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361705/ https://www.ncbi.nlm.nih.gov/pubmed/25775089 http://dx.doi.org/10.1371/journal.pone.0119401 |
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author | Canduela, Miren-Josune Mendizabal-Zubiaga, Juan Puente, Nagore Reguero, Leire Elezgarai, Izaskun Ramos-Uriarte, Almudena Gerrikagoitia, Inmaculada Grandes, Pedro |
author_facet | Canduela, Miren-Josune Mendizabal-Zubiaga, Juan Puente, Nagore Reguero, Leire Elezgarai, Izaskun Ramos-Uriarte, Almudena Gerrikagoitia, Inmaculada Grandes, Pedro |
author_sort | Canduela, Miren-Josune |
collection | PubMed |
description | We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (∼60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present. |
format | Online Article Text |
id | pubmed-4361705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43617052015-03-23 Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus Canduela, Miren-Josune Mendizabal-Zubiaga, Juan Puente, Nagore Reguero, Leire Elezgarai, Izaskun Ramos-Uriarte, Almudena Gerrikagoitia, Inmaculada Grandes, Pedro PLoS One Research Article We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (∼60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present. Public Library of Science 2015-03-16 /pmc/articles/PMC4361705/ /pubmed/25775089 http://dx.doi.org/10.1371/journal.pone.0119401 Text en © 2015 Canduela et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Canduela, Miren-Josune Mendizabal-Zubiaga, Juan Puente, Nagore Reguero, Leire Elezgarai, Izaskun Ramos-Uriarte, Almudena Gerrikagoitia, Inmaculada Grandes, Pedro Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
title | Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
title_full | Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
title_fullStr | Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
title_full_unstemmed | Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
title_short | Visualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyrus |
title_sort | visualization by high resolution immunoelectron microscopy of the transient receptor potential vanilloid-1 at inhibitory synapses of the mouse dentate gyrus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361705/ https://www.ncbi.nlm.nih.gov/pubmed/25775089 http://dx.doi.org/10.1371/journal.pone.0119401 |
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