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A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes

Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing flui...

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Autores principales: Strobl, Florian G, Breyer, Dominik, Link, Phillip, Torrano, Adriano A, Bräuchle, Christoph, Schneider, Matthias F, Wixforth, Achim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362322/
https://www.ncbi.nlm.nih.gov/pubmed/25821681
http://dx.doi.org/10.3762/bjnano.6.41
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author Strobl, Florian G
Breyer, Dominik
Link, Phillip
Torrano, Adriano A
Bräuchle, Christoph
Schneider, Matthias F
Wixforth, Achim
author_facet Strobl, Florian G
Breyer, Dominik
Link, Phillip
Torrano, Adriano A
Bräuchle, Christoph
Schneider, Matthias F
Wixforth, Achim
author_sort Strobl, Florian G
collection PubMed
description Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 μL can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO(2) particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms.
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spelling pubmed-43623222015-03-27 A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes Strobl, Florian G Breyer, Dominik Link, Phillip Torrano, Adriano A Bräuchle, Christoph Schneider, Matthias F Wixforth, Achim Beilstein J Nanotechnol Full Research Paper Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 μL can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO(2) particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms. Beilstein-Institut 2015-02-09 /pmc/articles/PMC4362322/ /pubmed/25821681 http://dx.doi.org/10.3762/bjnano.6.41 Text en Copyright © 2015, Strobl et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms)
spellingShingle Full Research Paper
Strobl, Florian G
Breyer, Dominik
Link, Phillip
Torrano, Adriano A
Bräuchle, Christoph
Schneider, Matthias F
Wixforth, Achim
A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
title A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
title_full A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
title_fullStr A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
title_full_unstemmed A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
title_short A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
title_sort surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362322/
https://www.ncbi.nlm.nih.gov/pubmed/25821681
http://dx.doi.org/10.3762/bjnano.6.41
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