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A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing flui...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Beilstein-Institut
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362322/ https://www.ncbi.nlm.nih.gov/pubmed/25821681 http://dx.doi.org/10.3762/bjnano.6.41 |
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author | Strobl, Florian G Breyer, Dominik Link, Phillip Torrano, Adriano A Bräuchle, Christoph Schneider, Matthias F Wixforth, Achim |
author_facet | Strobl, Florian G Breyer, Dominik Link, Phillip Torrano, Adriano A Bräuchle, Christoph Schneider, Matthias F Wixforth, Achim |
author_sort | Strobl, Florian G |
collection | PubMed |
description | Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 μL can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO(2) particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms. |
format | Online Article Text |
id | pubmed-4362322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Beilstein-Institut |
record_format | MEDLINE/PubMed |
spelling | pubmed-43623222015-03-27 A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes Strobl, Florian G Breyer, Dominik Link, Phillip Torrano, Adriano A Bräuchle, Christoph Schneider, Matthias F Wixforth, Achim Beilstein J Nanotechnol Full Research Paper Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 μL can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO(2) particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms. Beilstein-Institut 2015-02-09 /pmc/articles/PMC4362322/ /pubmed/25821681 http://dx.doi.org/10.3762/bjnano.6.41 Text en Copyright © 2015, Strobl et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms) |
spellingShingle | Full Research Paper Strobl, Florian G Breyer, Dominik Link, Phillip Torrano, Adriano A Bräuchle, Christoph Schneider, Matthias F Wixforth, Achim A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
title | A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
title_full | A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
title_fullStr | A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
title_full_unstemmed | A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
title_short | A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
title_sort | surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362322/ https://www.ncbi.nlm.nih.gov/pubmed/25821681 http://dx.doi.org/10.3762/bjnano.6.41 |
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