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Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.)
Introduction and exploitation of plant growth promoting rhizobacteria (PGPR) in agro-ecosystems enhance plant–microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. The present study was conducted to isolate and identify PGPRs associate...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362393/ https://www.ncbi.nlm.nih.gov/pubmed/25852667 http://dx.doi.org/10.3389/fmicb.2015.00207 |
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author | Zahid, Mahwish Abbasi, M. Kaleem Hameed, Sohail Rahim, Nasir |
author_facet | Zahid, Mahwish Abbasi, M. Kaleem Hameed, Sohail Rahim, Nasir |
author_sort | Zahid, Mahwish |
collection | PubMed |
description | Introduction and exploitation of plant growth promoting rhizobacteria (PGPR) in agro-ecosystems enhance plant–microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. The present study was conducted to isolate and identify PGPRs associated with maize (Zea mays L.) from twenty sites of Himalayan region of Hajira-Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. A total of 100 isolates were isolated from these sites, out of which eight (HJR(1), HJR(2), HJR(3), HJR(4), HJR(5), MR(6), HJR(7), HJR(8)) were selected in vitro for their plant growth promoting ability (PGPA) including phosphorus solubilization, indole-3-acetic acid (IAA) production and N(2) fixation. The 16S rRNA gene sequencing technique was used for molecular identity and authentication. Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions. The 16S rRNA gene sequencing and phylogenetic analysis identified these isolates belong to Pseudomonas and Bacillus genera. The isolates promoted plant growth by solubilizing soil P which ranged between 19.2 and 35.6 μg mL(-1). The isolates HJR(1), HJR(2), HJR(3), and HJR(5) showed positive activity in acetylene reduction assay showing their N(2)-fixation potential. All eight isolates showed the potential to produce IAA in the range of 0.9–5.39 μg mL(-1) and promote plant growth. Results from a subsequent pot experiment indicated PGPRs distinctly increased maize shoot and root length, shoot and root dry weight, root surface area, leaf surface area, shoot and root N and P contents. Among the eight isolates, HR(3) showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies. |
format | Online Article Text |
id | pubmed-4362393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43623932015-04-07 Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) Zahid, Mahwish Abbasi, M. Kaleem Hameed, Sohail Rahim, Nasir Front Microbiol Microbiology Introduction and exploitation of plant growth promoting rhizobacteria (PGPR) in agro-ecosystems enhance plant–microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. The present study was conducted to isolate and identify PGPRs associated with maize (Zea mays L.) from twenty sites of Himalayan region of Hajira-Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. A total of 100 isolates were isolated from these sites, out of which eight (HJR(1), HJR(2), HJR(3), HJR(4), HJR(5), MR(6), HJR(7), HJR(8)) were selected in vitro for their plant growth promoting ability (PGPA) including phosphorus solubilization, indole-3-acetic acid (IAA) production and N(2) fixation. The 16S rRNA gene sequencing technique was used for molecular identity and authentication. Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions. The 16S rRNA gene sequencing and phylogenetic analysis identified these isolates belong to Pseudomonas and Bacillus genera. The isolates promoted plant growth by solubilizing soil P which ranged between 19.2 and 35.6 μg mL(-1). The isolates HJR(1), HJR(2), HJR(3), and HJR(5) showed positive activity in acetylene reduction assay showing their N(2)-fixation potential. All eight isolates showed the potential to produce IAA in the range of 0.9–5.39 μg mL(-1) and promote plant growth. Results from a subsequent pot experiment indicated PGPRs distinctly increased maize shoot and root length, shoot and root dry weight, root surface area, leaf surface area, shoot and root N and P contents. Among the eight isolates, HR(3) showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies. Frontiers Media S.A. 2015-03-17 /pmc/articles/PMC4362393/ /pubmed/25852667 http://dx.doi.org/10.3389/fmicb.2015.00207 Text en Copyright © 2015 Zahid, Abbasi, Hameed and Rahim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zahid, Mahwish Abbasi, M. Kaleem Hameed, Sohail Rahim, Nasir Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) |
title | Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) |
title_full | Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) |
title_fullStr | Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) |
title_full_unstemmed | Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) |
title_short | Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.) |
title_sort | isolation and identification of indigenous plant growth promoting rhizobacteria from himalayan region of kashmir and their effect on improving growth and nutrient contents of maize (zea mays l.) |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362393/ https://www.ncbi.nlm.nih.gov/pubmed/25852667 http://dx.doi.org/10.3389/fmicb.2015.00207 |
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