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Centrin2 regulates CP110 removal in primary cilium formation

Primary cilia are antenna-like sensory microtubule structures that extend from basal bodies, plasma membrane–docked mother centrioles. Cellular quiescence potentiates ciliogenesis, but the regulation of basal body formation is not fully understood. We used reverse genetics to test the role of the sm...

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Detalles Bibliográficos
Autores principales: Prosser, Suzanna L., Morrison, Ciaran G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362459/
https://www.ncbi.nlm.nih.gov/pubmed/25753040
http://dx.doi.org/10.1083/jcb.201411070
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author Prosser, Suzanna L.
Morrison, Ciaran G.
author_facet Prosser, Suzanna L.
Morrison, Ciaran G.
author_sort Prosser, Suzanna L.
collection PubMed
description Primary cilia are antenna-like sensory microtubule structures that extend from basal bodies, plasma membrane–docked mother centrioles. Cellular quiescence potentiates ciliogenesis, but the regulation of basal body formation is not fully understood. We used reverse genetics to test the role of the small calcium-binding protein, centrin2, in ciliogenesis. Primary cilia arise in most cell types but have not been described in lymphocytes. We show here that serum starvation of transformed, cultured B and T cells caused primary ciliogenesis. Efficient ciliogenesis in chicken DT40 B lymphocytes required centrin2. We disrupted CETN2 in human retinal pigmented epithelial cells, and despite having intact centrioles, they were unable to make cilia upon serum starvation, showing abnormal localization of distal appendage proteins and failing to remove the ciliation inhibitor CP110. Knockdown of CP110 rescued ciliation in CETN2-deficient cells. Thus, centrin2 regulates primary ciliogenesis through controlling CP110 levels.
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spelling pubmed-43624592015-09-16 Centrin2 regulates CP110 removal in primary cilium formation Prosser, Suzanna L. Morrison, Ciaran G. J Cell Biol Research Articles Primary cilia are antenna-like sensory microtubule structures that extend from basal bodies, plasma membrane–docked mother centrioles. Cellular quiescence potentiates ciliogenesis, but the regulation of basal body formation is not fully understood. We used reverse genetics to test the role of the small calcium-binding protein, centrin2, in ciliogenesis. Primary cilia arise in most cell types but have not been described in lymphocytes. We show here that serum starvation of transformed, cultured B and T cells caused primary ciliogenesis. Efficient ciliogenesis in chicken DT40 B lymphocytes required centrin2. We disrupted CETN2 in human retinal pigmented epithelial cells, and despite having intact centrioles, they were unable to make cilia upon serum starvation, showing abnormal localization of distal appendage proteins and failing to remove the ciliation inhibitor CP110. Knockdown of CP110 rescued ciliation in CETN2-deficient cells. Thus, centrin2 regulates primary ciliogenesis through controlling CP110 levels. The Rockefeller University Press 2015-03-16 /pmc/articles/PMC4362459/ /pubmed/25753040 http://dx.doi.org/10.1083/jcb.201411070 Text en © 2015 Prosser and Morrison This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Prosser, Suzanna L.
Morrison, Ciaran G.
Centrin2 regulates CP110 removal in primary cilium formation
title Centrin2 regulates CP110 removal in primary cilium formation
title_full Centrin2 regulates CP110 removal in primary cilium formation
title_fullStr Centrin2 regulates CP110 removal in primary cilium formation
title_full_unstemmed Centrin2 regulates CP110 removal in primary cilium formation
title_short Centrin2 regulates CP110 removal in primary cilium formation
title_sort centrin2 regulates cp110 removal in primary cilium formation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362459/
https://www.ncbi.nlm.nih.gov/pubmed/25753040
http://dx.doi.org/10.1083/jcb.201411070
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