Cargando…
Centrin2 regulates CP110 removal in primary cilium formation
Primary cilia are antenna-like sensory microtubule structures that extend from basal bodies, plasma membrane–docked mother centrioles. Cellular quiescence potentiates ciliogenesis, but the regulation of basal body formation is not fully understood. We used reverse genetics to test the role of the sm...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362459/ https://www.ncbi.nlm.nih.gov/pubmed/25753040 http://dx.doi.org/10.1083/jcb.201411070 |
_version_ | 1782361814868164608 |
---|---|
author | Prosser, Suzanna L. Morrison, Ciaran G. |
author_facet | Prosser, Suzanna L. Morrison, Ciaran G. |
author_sort | Prosser, Suzanna L. |
collection | PubMed |
description | Primary cilia are antenna-like sensory microtubule structures that extend from basal bodies, plasma membrane–docked mother centrioles. Cellular quiescence potentiates ciliogenesis, but the regulation of basal body formation is not fully understood. We used reverse genetics to test the role of the small calcium-binding protein, centrin2, in ciliogenesis. Primary cilia arise in most cell types but have not been described in lymphocytes. We show here that serum starvation of transformed, cultured B and T cells caused primary ciliogenesis. Efficient ciliogenesis in chicken DT40 B lymphocytes required centrin2. We disrupted CETN2 in human retinal pigmented epithelial cells, and despite having intact centrioles, they were unable to make cilia upon serum starvation, showing abnormal localization of distal appendage proteins and failing to remove the ciliation inhibitor CP110. Knockdown of CP110 rescued ciliation in CETN2-deficient cells. Thus, centrin2 regulates primary ciliogenesis through controlling CP110 levels. |
format | Online Article Text |
id | pubmed-4362459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43624592015-09-16 Centrin2 regulates CP110 removal in primary cilium formation Prosser, Suzanna L. Morrison, Ciaran G. J Cell Biol Research Articles Primary cilia are antenna-like sensory microtubule structures that extend from basal bodies, plasma membrane–docked mother centrioles. Cellular quiescence potentiates ciliogenesis, but the regulation of basal body formation is not fully understood. We used reverse genetics to test the role of the small calcium-binding protein, centrin2, in ciliogenesis. Primary cilia arise in most cell types but have not been described in lymphocytes. We show here that serum starvation of transformed, cultured B and T cells caused primary ciliogenesis. Efficient ciliogenesis in chicken DT40 B lymphocytes required centrin2. We disrupted CETN2 in human retinal pigmented epithelial cells, and despite having intact centrioles, they were unable to make cilia upon serum starvation, showing abnormal localization of distal appendage proteins and failing to remove the ciliation inhibitor CP110. Knockdown of CP110 rescued ciliation in CETN2-deficient cells. Thus, centrin2 regulates primary ciliogenesis through controlling CP110 levels. The Rockefeller University Press 2015-03-16 /pmc/articles/PMC4362459/ /pubmed/25753040 http://dx.doi.org/10.1083/jcb.201411070 Text en © 2015 Prosser and Morrison This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Prosser, Suzanna L. Morrison, Ciaran G. Centrin2 regulates CP110 removal in primary cilium formation |
title | Centrin2 regulates CP110 removal in primary cilium formation |
title_full | Centrin2 regulates CP110 removal in primary cilium formation |
title_fullStr | Centrin2 regulates CP110 removal in primary cilium formation |
title_full_unstemmed | Centrin2 regulates CP110 removal in primary cilium formation |
title_short | Centrin2 regulates CP110 removal in primary cilium formation |
title_sort | centrin2 regulates cp110 removal in primary cilium formation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362459/ https://www.ncbi.nlm.nih.gov/pubmed/25753040 http://dx.doi.org/10.1083/jcb.201411070 |
work_keys_str_mv | AT prossersuzannal centrin2regulatescp110removalinprimaryciliumformation AT morrisonciarang centrin2regulatescp110removalinprimaryciliumformation |