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Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.

Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, β-mannana...

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Autores principales: Cameron, Hamish, Campion, Sylke H., Singh, Tripti, Vaidya, Alankar A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362734/
https://www.ncbi.nlm.nih.gov/pubmed/28324577
http://dx.doi.org/10.1007/s13205-014-0212-2
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author Cameron, Hamish
Campion, Sylke H.
Singh, Tripti
Vaidya, Alankar A.
author_facet Cameron, Hamish
Campion, Sylke H.
Singh, Tripti
Vaidya, Alankar A.
author_sort Cameron, Hamish
collection PubMed
description Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, β-mannanase. A Penicillium sp. was found to produce β-mannanase in both solid (31.6 units/g of dry biomass) and liquid (117 units/g of dry biomass) cultures using locust bean gum as an inducer after 2 weeks of incubation. The saccharification of steam-exploded Pinus radiata was 7.8 % w/w improved when the crude extract of Penicillium sp. was added to a mixture of commercial enzymes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-014-0212-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-43627342015-03-24 Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp. Cameron, Hamish Campion, Sylke H. Singh, Tripti Vaidya, Alankar A. 3 Biotech Short Report Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, β-mannanase. A Penicillium sp. was found to produce β-mannanase in both solid (31.6 units/g of dry biomass) and liquid (117 units/g of dry biomass) cultures using locust bean gum as an inducer after 2 weeks of incubation. The saccharification of steam-exploded Pinus radiata was 7.8 % w/w improved when the crude extract of Penicillium sp. was added to a mixture of commercial enzymes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-014-0212-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2014-04-06 2015-04 /pmc/articles/PMC4362734/ /pubmed/28324577 http://dx.doi.org/10.1007/s13205-014-0212-2 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ This article is published under license to BioMed Central Ltd. Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Short Report
Cameron, Hamish
Campion, Sylke H.
Singh, Tripti
Vaidya, Alankar A.
Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
title Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
title_full Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
title_fullStr Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
title_full_unstemmed Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
title_short Improved saccharification of steam-exploded Pinus radiata on supplementing crude extract of Penicillium sp.
title_sort improved saccharification of steam-exploded pinus radiata on supplementing crude extract of penicillium sp.
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362734/
https://www.ncbi.nlm.nih.gov/pubmed/28324577
http://dx.doi.org/10.1007/s13205-014-0212-2
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